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Literature summary for extracted from

  • Cristea, M.; Oliw, E.H.
    A G316A mutation of manganese lipoxygenase augments hydroperoxide isomerase activity: mechanism of biosynthesis of epoxyalcohols (2006), J. Biol. Chem., 281, 17612-17623.
    View publication on PubMed


Cloned (Comment) Organism
expressed in Pichia pastoris Gaeumannomyces graminis

Protein Variants

Protein Variants Comment Organism
G316A catalytically active, 7-8% of hydroperoxide isomerase activity Gaeumannomyces graminis
G316T catalytically inactive Gaeumannomyces graminis
G316V catalytically inactive Gaeumannomyces graminis


Inhibitors Comment Organism Structure
eicosatetraynoic acid 0.1 mM, 75% inhibition Gaeumannomyces graminis
N-(3-phenoxycinnamyl) acetohydroxamic acid 0.3 mM, complete inhibition Gaeumannomyces graminis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
(13R)-hydroperoxylinolenic acid mutant enzyme G316A Gaeumannomyces graminis


Metals/Ions Comment Organism Structure
Gaeumannomyces graminis


Organism UniProt Comment Textmining
Gaeumannomyces graminis Q8X151 variant avenae

Purification (Commentary)

Purification (Comment) Organism
phenyl-Sepharose column chromatography Gaeumannomyces graminis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(13R)-hydroperoxylinolenic acid + O2 only mutant enzyme G316A Gaeumannomyces graminis 13-ketolinolenic acid + epoxyalcohols erythro- and threo-11-hydroxy-(12R,13R)-epoxy-(9Z,15Z)-octadecadienoic acids and one of the corresponding cis-epoxides as major products ?
linoleic acid + O2
Gaeumannomyces graminis (9Z,11E)-(13R)-hydroperoxyoctadecadienoic acid


Synonyms Comment Organism
Gaeumannomyces graminis