Literature summary for 1.13.11.41 extracted from

Guo, J.; Erskine, P.; Coker, A.; Gor, J.; Perkins, S.; Wood, S.; Cooper, J.
Extension of resolution and oligomerization-state studies of 2,4'-dihydroxyacetophenone dioxygenase from Alcaligenes sp. 4HAP (2015), Acta Crystallogr. Sect. F, 71, 1258-1263 .

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Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapour diffusion method, mixing of 5 mg/ml protein in 50 mM Tris, pH 7.3, 100 mM NaCl, and mM 4-hydroxybenzoic acid or 0.1 mM 4-aminobenzamidine, with reservoir solution containing 0.1 M sodium cacodylate pH 5.9-6.8 and 1.1-1.6 M sodium acetate, method optimization, X-ray diffraction structure determination and analysis at 1.88 A resolution. The use of limited chymotrypsinolysis, which apparently results in removal of the first 20 or so N-terminal residues of DAD, is necessary for crystallization of the protein	Alcaligenes sp.

Crystallization (Comment)

Organism

hanging drop vapour diffusion method, mixing of 5 mg/ml protein in 50 mM Tris, pH 7.3, 100 mM NaCl, and mM 4-hydroxybenzoic acid or 0.1 mM 4-aminobenzamidine, with reservoir solution containing 0.1 M sodium cacodylate pH 5.9-6.8 and 1.1-1.6 M sodium acetate, method optimization, X-ray diffraction structure determination and analysis at 1.88 A resolution. The use of limited chymotrypsinolysis, which apparently results in removal of the first 20 or so N-terminal residues of DAD, is necessary for crystallization of the protein

Alcaligenes sp.

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
40000	-	recombinant chymotrypsinolysed enzyme, gel filtration and analytical ultracentrifugation	Alcaligenes sp.
80000	-	recombinant wild-type enzyme, gel filtration and analytical ultracentrifugation	Alcaligenes sp.

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2,4'-dihydroxyacetophenone + O2	Alcaligenes sp.	-	4-hydroxybenzoate + formate	-	?
additional information	Alcaligenes sp.	enzyme DAD is very unusual in that it is involved in C-C bond cleavage in a substituent of the aromatic ring instead of cleaving within the aromatic ring of the substrate	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Alcaligenes sp.	Q9REI7	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2,4'-dihydroxyacetophenone + O2	-	Alcaligenes sp.	4-hydroxybenzoate + formate	-	?
additional information	enzyme DAD is very unusual in that it is involved in C-C bond cleavage in a substituent of the aromatic ring instead of cleaving within the aromatic ring of the substrate	Alcaligenes sp.	?	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 20300, recombinant chymotrypsinolysed DAD, SDS-PAGE	Alcaligenes sp.
tetramer	4 * 20300, recombinant wild-type enzyme, SDS-PAGE	Alcaligenes sp.

Synonyms

Synonyms	Comment	Organism
DAD	-	Alcaligenes sp.