Cloned (Comment) | Organism |
---|---|
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Alcaligenes sp. |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged chymotrypsinolysed enzyme, 5 mg/ml protein in 50 mM Tris, pH 7.5, 100 mM NaCl, 1 mM 2-mercaptoethanol is mixed with a solution containing 10% w/v PEG 1000 and 10% PEG 8000, X-ray diffraction structure determination and analysis at 2.2 A resolution. The use of limited chymotrypsinolysis, which apparently results in removal of the first 20 or so N-terminal residues of DAD, is necessary for crystallization of the protein | Alcaligenes sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | one non-heme iron per enzyme subunit. The catalytic iron is coordinated by three histidine residues, H76, H78 and H114 in strands II and VII, within a buried active-site cavity, and an additional ligand | Alcaligenes sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4'-dihydroxyacetophenone + O2 | Alcaligenes sp. | - |
4-hydroxybenzoate + formate | - |
? | |
additional information | Alcaligenes sp. | enzyme DAD is very unusual in that it is involved in C-C bond cleavage in a substituent of the aromatic ring instead of cleaving within the aromatic ring of the substrate | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alcaligenes sp. | Q9REI7 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Alcaligenes sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4'-dihydroxyacetophenone + O2 | - |
Alcaligenes sp. | 4-hydroxybenzoate + formate | - |
? | |
additional information | enzyme DAD is very unusual in that it is involved in C-C bond cleavage in a substituent of the aromatic ring instead of cleaving within the aromatic ring of the substrate | Alcaligenes sp. | ? | - |
? | |
additional information | modeling of substrate binding in the active site, overview | Alcaligenes sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | enzyme tertiary and quarternary structure analysis | Alcaligenes sp. |
tetramer | 4 * 20300, recombinant wild-type enzyme, SDS-PAGE | Alcaligenes sp. |
Synonyms | Comment | Organism |
---|---|---|
DAD | - |
Alcaligenes sp. |
General Information | Comment | Organism |
---|---|---|
additional information | active site structure and substrate binding structure, modeling, overview | Alcaligenes sp. |