Cloned (Comment) | Organism |
---|---|
skin 8-lipoxygenase expressed in COS-7 cells by transient transfection of its cDNA, amplified by PCR, in pEF-BOS, also expressed in Escherichia coli system using pQE-32 | Mus musculus |
General Stability | Organism |
---|---|
leukotriene A synthase activity of the enzyme leveled off within 10 min, indicating suicide activation | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AA861 | - |
Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 arachidonic acid + 2 O2 | Mus musculus | - |
8-hydroxy,9-oxo-eicosa-5Z,11Z,14Z-trienoic acid + 9-oxo-[8,12-cis]-prosta-5Z,10,14Z-trienoic acid + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
skin | - |
Mus musculus | - |
Storage Stability | Organism |
---|---|
-70°C, crude and purified enzyme preparations can be stored without appreciable loss of activity for at least 6 months | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 arachidonic acid + 2 O2 | - |
Mus musculus | 8-hydroxy,9-oxo-eicosa-5Z,11Z,14Z-trienoic acid + 9-oxo-[8,12-cis]-prosta-5Z,10,14Z-trienoic acid + H2O | - |
? | |
5-hydroperoxy fatty acid + O2 | leukotriene A synthase activity, reaction rate is approximately 7% of arachidonate 8-lipoxygenation | Mus musculus | leukotriene A4 | - |
? | |
5-hydroperoxyeicosatetraenoic acid + ? | - |
Mus musculus | leukotriene A4 | unstable product | ? | |
alpha-linolenic acid + O2 | - |
Mus musculus | ? | - |
? | |
arachidonic acid + O2 | - |
Mus musculus | (8R)-8-hydroperoxy-5,9,11,14-eicosatetraenoic acid | - |
? | |
docosahexaenoic acid + O2 | - |
Mus musculus | ? | - |
? | |
eicosapentaenoic acid + O2 | - |
Mus musculus | ? | - |
? | |
gamma-linoleic acid + O2 | - |
Mus musculus | ? | - |
? | |
linoleic acid + O2 | poor substrate | Mus musculus | ? | - |
? |