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Literature summary for 1.13.11.4 extracted from
- Biochemical and molecular characterization of a ring fission dioxygenase with the ability to oxidize (substituted) salicylate(s) from Pseudaminobacter salicylatoxidans (2004), J. Biol. Chem., 279, 37250-37260 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| heterologously expressed in Escherichia coli as His-tagged enzyme | Pseudaminobacter salicylatoxidans |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.012 | - |
salicylate | pH 8.0, temperature not specified in the publication | Pseudaminobacter salicylatoxidans |  |
| 0.027 | - |
3-chlorosalicylate | pH 8.0, temperature not specified in the publication | Pseudaminobacter salicylatoxidans | |
| 0.028 | - |
4-methylsalicylate | pH 8.0, temperature not specified in the publication | Pseudaminobacter salicylatoxidans | |
| 0.039 | - |
4-hydroxysalicylate | pH 8.0, temperature not specified in the publication | Pseudaminobacter salicylatoxidans | |
| 0.067 | - |
3-hydroxysalicylate | pH 8.0, temperature not specified in the publication | Pseudaminobacter salicylatoxidans | |
| 0.071 | - |
3-bromosalicylate | pH 8.0, temperature not specified in the publication | Pseudaminobacter salicylatoxidans | |
| 0.079 | - |
gentisate | pH 8.0, temperature not specified in the publication | Pseudaminobacter salicylatoxidans | |
| 0.085 | - |
5-chlorosalicylate | pH 8.0, temperature not specified in the publication | Pseudaminobacter salicylatoxidans | |
| 0.091 | - |
5-Fluorosalicylate | pH 8.0, temperature not specified in the publication | Pseudaminobacter salicylatoxidans | |
| 0.1 | - |
4-chlorosalicylate | pH 8.0, temperature not specified in the publication | Pseudaminobacter salicylatoxidans | |
| 0.116 | - |
3-methylsalicylate | pH 8.0, temperature not specified in the publication | Pseudaminobacter salicylatoxidans | |
| 0.328 | - |
5-Methylsalicylate | pH 8.0, temperature not specified in the publication | Pseudaminobacter salicylatoxidans | |
| 2.3 | - |
3-aminosalicylate | pH 8.0, temperature not specified in the publication | Pseudaminobacter salicylatoxidans | |
| 3.1 | - |
5-Aminosalicylate | pH 8.0, temperature not specified in the publication | Pseudaminobacter salicylatoxidans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41176 | - |
calculated from sequence | Pseudaminobacter salicylatoxidans |
| 45000 | - |
SDS-PAGE | Pseudaminobacter salicylatoxidans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudaminobacter salicylatoxidans | Q67FT0 | - |
- |
| Pseudaminobacter salicylatoxidans BN12 | Q67FT0 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudaminobacter salicylatoxidans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,5-dihydroxybenzoate + O2 | i.e. gentisate | Pseudaminobacter salicylatoxidans | maleylpyruvate | - |
? | |
| 2,5-dihydroxybenzoate + O2 | i.e. gentisate | Pseudaminobacter salicylatoxidans BN12 | maleylpyruvate | - |
? | |
| 3-aminosalicylate + O2 | - |
Pseudaminobacter salicylatoxidans | (2E,4Z)-2-amino-6-oxohepta-2,4-dienoate | - |
? | |
| 3-bromosalicylate + O2 | low activity | Pseudaminobacter salicylatoxidans | (2E,4Z)-2-bromo-6-oxohepta-2,4-dienoate | - |
? | |
| 3-chlorosalicylate + O2 | low activity | Pseudaminobacter salicylatoxidans | (2E,4Z)-2-chloro-6-oxohepta-2,4-dienoate | - |
? | |
| 3-hydroxysalicylate + O2 | low activity | Pseudaminobacter salicylatoxidans | (2E,4Z)-2-hydroxy-6-oxohepta-2,4-dienoate | - |
? | |
| 3-methylsalicylate + O2 | low activity | Pseudaminobacter salicylatoxidans | (2Z,4Z)-2-methyl-6-oxohepta-2,4-dienoate | - |
? | |
| 4-aminosalicylate + O2 | - |
Pseudaminobacter salicylatoxidans | (2Z,4E)-3-amino-6-oxohepta-2,4-dienoate | - |
? | |
| 4-chlorosalicylate + O2 | low activity | Pseudaminobacter salicylatoxidans | (2Z,4E)-3-chloro-6-oxohepta-2,4-dienoate | - |
? | |
| 4-hydroxysalicylate + O2 | low activity | Pseudaminobacter salicylatoxidans | (2Z,4E)-3-hydroxy-6-oxohepta-2,4-dienoate | - |
? | |
| 4-methylsalicylate + O2 | low activity | Pseudaminobacter salicylatoxidans | (2Z,4Z)-3-methyl-6-oxohepta-2,4-dienoate | - |
? | |
| 5-aminosalicylate + O2 | - |
Pseudaminobacter salicylatoxidans | (2Z,4E)-4-amino-6-oxohepta-2,4-dienedioate | - |
? | |
| 5-aminosalicylate + O2 | - |
Pseudaminobacter salicylatoxidans BN12 | (2Z,4E)-4-amino-6-oxohepta-2,4-dienedioate | - |
? | |
| 5-chlorosalicylate + O2 | low activity | Pseudaminobacter salicylatoxidans | (2Z,4E)-4-chloro-6-oxohepta-2,4-dienoate | - |
? | |
| 5-fluorosalicylate + O2 | low activity | Pseudaminobacter salicylatoxidans | (2Z,4E)-4-fluoro-6-oxohepta-2,4-dienoate | - |
? | |
| 5-methylsalicylate + O2 | low activity | Pseudaminobacter salicylatoxidans | (2Z,4Z)-4-methyl-6-oxohepta-2,4-dienoate | - |
? | |
| gentisate + O2 | - |
Pseudaminobacter salicylatoxidans | ? | - |
? | |
| gentisate + O2 | - |
Pseudaminobacter salicylatoxidans BN12 | ? | - |
? | |
| additional information | the purified enzyme is able to convert various salicylates that carried methyl-, fluoro-, chloro-, bromo-, or iodo-substituents in the 3-, 4-, or 5-position, although in general with much lower specific activities than observed with 5-amino- or 5-hydroxysalicylate. No activity with 6-hydroxysalicylate, 5-carboxysalicylate, 5-sulfosalicylate, 5-nitrosalicylate, 3,5-dibromosalicylate, 3,5-diiodosalicylate, or 3,4-dihydroxysalicylate | Pseudaminobacter salicylatoxidans | ? | - |
? | |
| additional information | the purified enzyme is able to convert various salicylates that carried methyl-, fluoro-, chloro-, bromo-, or iodo-substituents in the 3-, 4-, or 5-position, although in general with much lower specific activities than observed with 5-amino- or 5-hydroxysalicylate. No activity with 6-hydroxysalicylate, 5-carboxysalicylate, 5-sulfosalicylate, 5-nitrosalicylate, 3,5-dibromosalicylate, 3,5-diiodosalicylate, or 3,4-dihydroxysalicylate | Pseudaminobacter salicylatoxidans BN12 | ? | - |
? | |
| salicylate + O2 | - |
Pseudaminobacter salicylatoxidans | 2-oxohepta-3,5-dienedioic acid | - |
? | |
| salicylate + O2 | - |
Pseudaminobacter salicylatoxidans BN12 | 2-oxohepta-3,5-dienedioic acid | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 45000, SDS-PAGE | Pseudaminobacter salicylatoxidans |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
4-Aminosalicylate | pH 8.0, temperature not specified in the publication | Pseudaminobacter salicylatoxidans | |
| 0.2 | - |
4-hydroxysalicylate | pH 8.0, temperature not specified in the publication | Pseudaminobacter salicylatoxidans | |
| 0.3 | - |
4-methylsalicylate | pH 8.0, temperature not specified in the publication | Pseudaminobacter salicylatoxidans | |
| 0.3 | - |
3-bromosalicylate | pH 8.0, temperature not specified in the publication | Pseudaminobacter salicylatoxidans | |
| 0.4 | - |
3-chlorosalicylate | pH 8.0, temperature not specified in the publication | Pseudaminobacter salicylatoxidans | |
| 0.5 | - |
5-chlorosalicylate | pH 8.0, temperature not specified in the publication | Pseudaminobacter salicylatoxidans | |
| 0.5 | - |
3-hydroxysalicylate | pH 8.0, temperature not specified in the publication | Pseudaminobacter salicylatoxidans | |
| 0.8 | - |
4-chlorosalicylate | pH 8.0, temperature not specified in the publication | Pseudaminobacter salicylatoxidans | |
| 2.3 | - |
salicylate | pH 8.0, temperature not specified in the publication | Pseudaminobacter salicylatoxidans | |
| 2.9 | - |
3-methylsalicylate | pH 8.0, temperature not specified in the publication | Pseudaminobacter salicylatoxidans | |
| 3.6 | - |
5-Fluorosalicylate | pH 8.0, temperature not specified in the publication | Pseudaminobacter salicylatoxidans | |
| 4 | - |
3-aminosalicylate | pH 8.0, temperature not specified in the publication | Pseudaminobacter salicylatoxidans | |
| 14.3 | - |
5-Methylsalicylate | pH 8.0, temperature not specified in the publication | Pseudaminobacter salicylatoxidans | |
| 59.3 | - |
5-Aminosalicylate | pH 8.0, temperature not specified in the publication | Pseudaminobacter salicylatoxidans | |
| 225 | - |
gentisate | pH 8.0, temperature not specified in the publication | Pseudaminobacter salicylatoxidans | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.7 | - |
3-aminosalicylate | pH 8.0, temperature not specified in the publication | Pseudaminobacter salicylatoxidans | |
| 3.6 | - |
3-bromosalicylate | pH 8.0, temperature not specified in the publication | Pseudaminobacter salicylatoxidans | |
| 6.2 | - |
5-chlorosalicylate | pH 8.0, temperature not specified in the publication | Pseudaminobacter salicylatoxidans | |
| 6.2 | - |
4-hydroxysalicylate | pH 8.0, temperature not specified in the publication | Pseudaminobacter salicylatoxidans | |
| 7.1 | - |
3-hydroxysalicylate | pH 8.0, temperature not specified in the publication | Pseudaminobacter salicylatoxidans | |
| 8.1 | - |
4-chlorosalicylate | pH 8.0, temperature not specified in the publication | Pseudaminobacter salicylatoxidans | |
| 11 | - |
4-methylsalicylate | pH 8.0, temperature not specified in the publication | Pseudaminobacter salicylatoxidans | |
| 13.6 | - |
3-chlorosalicylate | pH 8.0, temperature not specified in the publication | Pseudaminobacter salicylatoxidans | |
| 19.1 | - |
5-Aminosalicylate | pH 8.0, temperature not specified in the publication | Pseudaminobacter salicylatoxidans | |
| 25 | - |
3-methylsalicylate | pH 8.0, temperature not specified in the publication | Pseudaminobacter salicylatoxidans | |
| 28.4 | - |
gentisate | pH 8.0, temperature not specified in the publication | Pseudaminobacter salicylatoxidans | |
| 39.3 | - |
5-Fluorosalicylate | pH 8.0, temperature not specified in the publication | Pseudaminobacter salicylatoxidans | |
| 43 | - |
5-Methylsalicylate | pH 8.0, temperature not specified in the publication | Pseudaminobacter salicylatoxidans | |
| 187.5 | - |
salicylate | pH 8.0, temperature not specified in the publication | Pseudaminobacter salicylatoxidans | |
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