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Literature summary for 1.13.11.4 extracted from

  • Liu, T.T.; Xu, Y.; Liu, H.; Luo, S.; Yin, Y.J.; Liu, S.J.; Zhou, N.Y.
    Functional characterization of a gene cluster involved in gentisate catabolism in Rhodococcus sp. strain NCIMB 12038 (2010), Appl. Microbiol. Biotechnol., 90, 671-678.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene narI, DNA and amino acid sequence determination and analysis of the gentisate catabolic gene cluster. Among the 13 complete open reading frames deduced from this fragment, three narIKL encode the enzymes involved in the reactions of gentisate catabolism, of these, NarI is gentisate 1,2-dioxygenase which converts gentisate to maleylpyruvate. Genes narI, narK, and narL genes are transcribed as a single operon only in the presence of naphthalene. Sequence comparisons, overview. Expression in Escherichia coli strain Rosetta (DE3) pLysS Rhodococcus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2,5-dihydroxybenzoate + O2 Rhodococcus sp.
-
maleylpyruvate
-
?
2,5-dihydroxybenzoate + O2 Rhodococcus sp. NCIMB 12038
-
maleylpyruvate
-
?

Organism

Organism UniProt Comment Textmining
Rhodococcus sp. E7CYQ0 gene narI
-
Rhodococcus sp. NCIMB 12038 E7CYQ0 gene narI
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information strain NCIMB 12038 utilizes naphthalene as a sole source of carbon and energy Rhodococcus sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
145
-
recombinant enzyme, pH 7.4, 30°C Rhodococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,5-dihydroxybenzoate + O2
-
Rhodococcus sp. maleylpyruvate
-
?
2,5-dihydroxybenzoate + O2
-
Rhodococcus sp. NCIMB 12038 maleylpyruvate
-
?

Synonyms

Synonyms Comment Organism
GDO
-
Rhodococcus sp.
NarI
-
Rhodococcus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Rhodococcus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Rhodococcus sp.

General Information

General Information Comment Organism
metabolism narI is involved in salicylate, i.e. 3-hydroxybenzoate, and gentisate catabolism, organization of the 3HBA-gentisate catabolic cluster, overview Rhodococcus sp.
additional information strain NCIMB 12038 utilizes naphthalene as a sole source of carbon and energy, and degrades naphthalene via salicylate and gentisate. The strain can also use salicylate, i.e. 3-hydroxybenzoate, as sole source of energy and carbon Rhodococcus sp.