Crystallization (Comment) | Organism |
---|---|
crystal structure at 2.8 A resolution, hanging drop vapor diffusion crystallization at 16°C. The crystal form belongs to the R32 space group, with two protein molecules (named as A and B) per asymmetric unit and 45% solvent content | Ruegeria pomeroyi |
Protein Variants | Comment | Organism |
---|---|---|
D175N | inactive mutant enzyme | Ruegeria pomeroyi |
D225N | activity is comparable to wild-type activity | Ruegeria pomeroyi |
E47Q | activity is comparable to wild-type activity | Ruegeria pomeroyi |
H119A/H121A | despite its wild-type like structural propertie, the mutant shows extremely low gentisate 1,2-dioxygenase activity | Ruegeria pomeroyi |
H290A/H292A | mutant can not be expressed in a soluble form | Ruegeria pomeroyi |
L39T | activity is comparable to wild-type activity | Ruegeria pomeroyi |
P253_Y254del | mutation reduces the activity to 30% | Ruegeria pomeroyi |
Q108E | inactive mutant enzyme | Ruegeria pomeroyi |
Q108E/D175N | activity is near zero | Ruegeria pomeroyi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
2,5-Dihydroxybenzoate | - |
Ruegeria pomeroyi |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | two ferrous centers are located in its two homologous cupin domains, respectively. The N-terminal Fe2+-binding site is essential for the enzyme activity but dispensable for the protein overall tertiary structure | Ruegeria pomeroyi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ruegeria pomeroyi | Q5LLB1 | DSS-3 | - |
Purification (Comment) | Organism |
---|---|
- |
Ruegeria pomeroyi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,5-dihydroxybenzoate + O2 | - |
Ruegeria pomeroyi | maleylpyruvate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GDOsp | - |
Ruegeria pomeroyi |
gtdA-2 | - |
Ruegeria pomeroyi |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
protein unfolds and loses its secondary structures above | Ruegeria pomeroyi |