Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion method using 0.1 M BisTris pH 6.5 and 20% polyethylene glycol 5000 monomethylether | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 5 mM, complete inhibition at pH 8.0 | Escherichia coli | |
EDTA | complete inhibition at pH 8.0 | Escherichia coli | |
Li2SO4 | 5 mM 5-10% inhibition at pH 8.0 | Escherichia coli | |
MgSO4 | 5 mM, 5-10% inhibition at pH 8.0 | Escherichia coli | |
Mn2+ | 5 mM, complete inhibition at pH 8.0 | Escherichia coli | |
additional information | no inhibition by 5 mM Ca2+ and K+ | Escherichia coli | |
Na2SO4 | 5 mM, 5-10% inhibition at pH 8.0 | Escherichia coli | |
Zn2+ | 5 mM, complete inhibition at pH 8.0 | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
2,5-Dihydroxybenzoate | - |
Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | contains 1 mol iron per subunit, in the presence of 2 mM Fe2+ activity is increased by 115% | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38500 | - |
4 * 38500, gel filtration | Escherichia coli |
38900 | - |
SDS-PAGE | Escherichia coli |
154000 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | Q8X655 | - |
- |
Purification (Comment) | Organism |
---|---|
nickel-nitriloacetic acid resin column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,5-dihydroxybenzoate + O2 | - |
Escherichia coli | maleylpyruvate | - |
? | |
additional information | methyl 2,5-dihydroxybenzoate, 1,4-benzoquinone, 2,5-dihydroxybenzaldehyde, 4,5-dihydroxy-1,3-benzene-disulphonic acid and 2,3,5,6-tetrachloro-1,4-benzenediol give no substrate activity | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 38500, gel filtration | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
eGDO | - |
Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | - |
Escherichia coli |