Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.13.11.4 extracted from

  • Werwath, J.; Arfmann, H.A.; Pieper, D.H.; Timmis, K.N.; Wittich, R.M.
    Biochemical and genetic characterization of a gentisate 1,2-dioxygenase from Sphingomonas sp. strain RW5 (1998), J. Bacteriol., 180, 4171-4176.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.11.4

Cloned(Commentary)

Cloned (Comment) Organism
-
 Sphingomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
 gentisate
-
 Sphingomonas sp.
0.754
-
 3,6-dichlorogentisate
-
 Sphingomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38500
-
 4 * 38500, SDS-PAGE, recombinant protein from Escherichia coli Sphingomonas sp.
177000
-
 gel filtration, recombinant protein from Escherichia coli Sphingomonas sp.

Organism

Organism UniProt Comment Textmining
Sphingomonas sp.
-
 RW5
-
Sphingomonas sp. RW5
-
 RW5
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein from Escherichia coli Sphingomonas sp.

Storage Stability

Storage Stability Organism
-70°C, 2 weeks, 70% loss of activity Sphingomonas sp.
4°C, 72 h, 90% loss of activity Sphingomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,5-dihydroxybenzoate + O2
-
 Sphingomonas sp. maleylpyruvate
-
 ?
2,5-dihydroxybenzoate + O2
-
 Sphingomonas sp. RW5 maleylpyruvate
-
 ?
3,6-dichlorogentisate + O2
-
 Sphingomonas sp. ?
-
 ?
3,6-dichlorogentisate + O2
-
 Sphingomonas sp. RW5 ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 38500, SDS-PAGE, recombinant protein from Escherichia coli Sphingomonas sp.