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Literature summary for 1.13.11.4 extracted from

  • Kiemer, P.; Tshisuaka, B.; Fetzner, S.; Lingens, F.
    Degradation of benzoate via benzoyl-coenzyme A and gentisate by Bacillus stearothermophilus PK1, and purification of gentisate 1,2-dioxygenase (1996), Biol. Fertil. Soils, 23, 307-313.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Fe2+ strictly dependent on Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
6 * 40000, SDS-PAGE Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
PK1
-
Geobacillus stearothermophilus PK1
-
PK1
-

Purification (Commentary)

Purification (Comment) Organism
-
Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,5-dihydroxybenzoate + O2
-
Geobacillus stearothermophilus maleylpyruvate
-
?
2,5-dihydroxybenzoate + O2
-
Geobacillus stearothermophilus PK1 maleylpyruvate
-
?

Subunits

Subunits Comment Organism
hexamer 6 * 40000, SDS-PAGE Geobacillus stearothermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65 70
-
Geobacillus stearothermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
80 min, 50% loss of activity Geobacillus stearothermophilus