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Literature summary for 1.13.11.4 extracted from

  • Suarez, M.; Ferrer, E.; Martin, M.
    Purification and biochemical characterization of gentisate 1,2-dioxygenase from Klebsiella pneumoniae M5a1 (1996), FEMS Microbiol. Lett., 143, 89-95.
    View publication on PubMed

General Stability

General Stability Organism
glycerol, beta-mercaptoethanol stabilize Klebsiella pneumoniae

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 1 mM, complete inactivation Klebsiella pneumoniae
EDTA 100 mM, 85% inactivation Klebsiella pneumoniae
Fe3+ 2 mM, complete inactivation Klebsiella pneumoniae
H2O2 3 mM, 90% inactivation Klebsiella pneumoniae
Mn2+ 10 mM, complete inactivation Klebsiella pneumoniae
Zn2+ 10 mM, complete inactivation Klebsiella pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.052
-
gentisate
-
Klebsiella pneumoniae

Metals/Ions

Metals/Ions Comment Organism Structure
Iron Fe2+, 0.9-1 atom per subunit Klebsiella pneumoniae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
4 * 38000, amino acid composition Klebsiella pneumoniae
159000
-
gel filtration Klebsiella pneumoniae

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Klebsiella pneumoniae

Renatured (Commentary)

Renatured (Comment) Organism
H2O2-inactivated enzyme is restored by hydroxylamine or ferrous iron Klebsiella pneumoniae

Storage Stability

Storage Stability Organism
-20°C, addition of glycerol and beta-mercaptoethanol, 96 h, 25% of activity remains Klebsiella pneumoniae
4°C, 48 h, complete loss of activity Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,5-dihydroxybenzoate + O2
-
Klebsiella pneumoniae maleylpyruvate
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 38000, amino acid composition Klebsiella pneumoniae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Klebsiella pneumoniae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9
-
Klebsiella pneumoniae