General Stability | Organism |
---|---|
glycerol, beta-mercaptoethanol stabilize | Klebsiella pneumoniae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 1 mM, complete inactivation | Klebsiella pneumoniae | |
EDTA | 100 mM, 85% inactivation | Klebsiella pneumoniae | |
Fe3+ | 2 mM, complete inactivation | Klebsiella pneumoniae | |
H2O2 | 3 mM, 90% inactivation | Klebsiella pneumoniae | |
Mn2+ | 10 mM, complete inactivation | Klebsiella pneumoniae | |
Zn2+ | 10 mM, complete inactivation | Klebsiella pneumoniae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.052 | - |
gentisate | - |
Klebsiella pneumoniae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | Fe2+, 0.9-1 atom per subunit | Klebsiella pneumoniae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
4 * 38000, amino acid composition | Klebsiella pneumoniae |
159000 | - |
gel filtration | Klebsiella pneumoniae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella pneumoniae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Klebsiella pneumoniae |
Renatured (Comment) | Organism |
---|---|
H2O2-inactivated enzyme is restored by hydroxylamine or ferrous iron | Klebsiella pneumoniae |
Storage Stability | Organism |
---|---|
-20°C, addition of glycerol and beta-mercaptoethanol, 96 h, 25% of activity remains | Klebsiella pneumoniae |
4°C, 48 h, complete loss of activity | Klebsiella pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,5-dihydroxybenzoate + O2 | - |
Klebsiella pneumoniae | maleylpyruvate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 38000, amino acid composition | Klebsiella pneumoniae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Klebsiella pneumoniae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | - |
Klebsiella pneumoniae |