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Literature summary for 1.13.11.39 extracted from
- Catalytic properties of 2,3-dihydroxybiphenyl 1,2-dioxygenase from Dyella ginsengisoli LA-4 immobilized on mesoporous silica SBA-15 (2014), J. Mol. Catal. B, 99, 136-142 .
No PubMed abstract available
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | immobilization of the enzyme BphC on mesoporous silica SBA-15 in order to improve its stability with relatively high retaining activities. By Fourier transformed infrared spectroscopy (FTIR) and N2 adsorption/desorption isotherms, BphC is confirmed to be successfully adsorbed and captured on SBA-15. Under the experimental conditions, the maximum loading amount reaches 124.6 mg protein/g support at pH 6.0. The immobilized BphC is more stable at 4°C than the free enzyme and the thermostability of BphC is significantly improved by immobilization compared to the wild-type enzyme, while the catalytic efficiency with biphenyl-2,3-diol is reduced. The activity and stability alteration occur due to the changes of secondary structure of BphC. Mechanism of immobilization | Dyella ginsengisoli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | Dyella ginsengisoli | |
| 0.00364 | - |
biphenyl-2,3-diol | pH 8.0, 30°C, free enzyme | Dyella ginsengisoli |  |
| 0.01022 | - |
biphenyl-2,3-diol | pH 8.0, 30°C, immobilized enzyme | Dyella ginsengisoli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | required | Dyella ginsengisoli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| biphenyl-2,3-diol + O2 | Dyella ginsengisoli | - |
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate | - |
? | |
| biphenyl-2,3-diol + O2 | Dyella ginsengisoli LA-4 | - |
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Dyella ginsengisoli | - |
- |
- |
| Dyella ginsengisoli LA-4 | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 10.74 | - |
pH 8.0, 30°C, immobilized enzyme, substrate biphenyl-2,3-diol | Dyella ginsengisoli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, purified immobilized enzyme BphC,16% of its initial activity after 5 days, the purified free enzyme loses its activity rapidly within 12 h | Dyella ginsengisoli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-methylcatechol + O2 | - |
Dyella ginsengisoli | 2-hydroxy-6-oxohepta-2,4-dienoate | - |
? | |
| 3-methylcatechol + O2 | - |
Dyella ginsengisoli LA-4 | 2-hydroxy-6-oxohepta-2,4-dienoate | - |
? | |
| biphenyl-2,3-diol + O2 | - |
Dyella ginsengisoli | 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate | - |
? | |
| biphenyl-2,3-diol + O2 | - |
Dyella ginsengisoli LA-4 | 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate | - |
? | |
| catechol + O2 | - |
Dyella ginsengisoli | 2-hydroxymuconate semialdehyde | - |
? | |
| catechol + O2 | - |
Dyella ginsengisoli LA-4 | 2-hydroxymuconate semialdehyde | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 2,3-dihydroxybiphenyl 1,2-dioxygenase | - |
Dyella ginsengisoli |
| BphC | - |
Dyella ginsengisoli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
free enzyme | Dyella ginsengisoli |
| 45 | - |
immobilized enzyme | Dyella ginsengisoli |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | 55 | activity range of immobilized enzyme | Dyella ginsengisoli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
purified immobilized BphC possess 90% of its initial activity after incubating for 4 h | Dyella ginsengisoli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 20.63 | - |
biphenyl-2,3-diol | pH 8.0, 30°C, immobilized enzyme | Dyella ginsengisoli | |
| 79.65 | - |
biphenyl-2,3-diol | pH 8.0, 30°C, free enzyme | Dyella ginsengisoli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
immobilized enzyme | Dyella ginsengisoli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | activity range of immobilized enzyme | Dyella ginsengisoli |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | enzyme structure molecular simulation, overview | Dyella ginsengisoli |
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