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Literature summary for 1.13.11.38 extracted from
- Biodegradation of phenanthrene by Pseudomonas sp. strain PPD: purification and characterization of 1-hydroxy-2-naphthoic acid dioxygenase (2009), Microbiology, 155, 3083-3091.
General Stability
| General Stability | Organism |
|---|---|
| the enzyme is very unstable in the cell-free extract and requires Fe(II) (0.1 mM), DTT (2 mM) and ethanol (10%) for stability | Pseudomonas sp. |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-hydroxy-2-naphthoic acid | substrate inhibition | Pseudomonas sp. |  |
| 3-Hydroxy-2-naphthoic acid | competitive inhibition | Pseudomonas sp. | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0135 | - |
1-hydroxy-2-naphthoate | - |
Pseudomonas sp. | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | - |
Pseudomonas sp. | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39000 | - |
SDS-PAGE under denaturing conditions, 4 * 39000 | Pseudomonas sp. |
| 160000 | - |
Sephacryl S-200-HR gel-filtration chromatography; SDS-PAGE under denaturing conditions, homotetramer, subunit molecular mass of about 39000 | Pseudomonas sp. |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-hydroxy-2-naphthoic acid + O2 | Pseudomonas sp. | - |
2-carboxybenzylpyruvic acid | - |
? | |
| additional information | Pseudomonas sp. | 1-hydroxy-2-naphthoic acid dioxygenase shows no activity with gentisic acid, salicylic acid and other hydroxynaphthoic acids | ? | - |
? | |
| additional information | Pseudomonas sp. | Pseudomonas sp. strain PPD can metabolize phenanthrene as the sole source of carbon and energy via the phthalic acid route | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas sp. | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity using a 3-hydroxy-2-naphthoic acid (3-H2NA)-affinity matrix, DEAE-anion-exchange chromatography | Pseudomonas sp. |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture condition:phenanthrene-grown cell | - |
Pseudomonas sp. | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.9 | - |
cell-free extract | Pseudomonas sp. |
| 11.2 | - |
heat treatment (60°C) | Pseudomonas sp. |
| 29 | - |
anion-exchange chromatography | Pseudomonas sp. |
| 53 | - |
3-H2NA-affinity chromatography | Pseudomonas sp. |
| 61.5 | - |
after all purification steps from Pseudomonas sp. PPD cells grown on phenanthrene | Pseudomonas sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-hydroxy-2-naphthoate + O2 | - |
Pseudomonas sp. | (3Z)-4-(2-carboxyphenyl)-2-oxobut-3-enoate | - |
r | |
| 1-hydroxy-2-naphthoic acid + O2 | - |
Pseudomonas sp. | 2-carboxybenzylpyruvic acid | - |
? | |
| additional information | 1-hydroxy-2-naphthoic acid dioxygenase shows no activity with gentisic acid, salicylic acid and other hydroxynaphthoic acids | Pseudomonas sp. | ? | - |
? | |
| additional information | Pseudomonas sp. strain PPD can metabolize phenanthrene as the sole source of carbon and energy via the phthalic acid route | Pseudomonas sp. | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | SDS-PAGE under denaturing conditions, 4 * 39000 | Pseudomonas sp. |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 1-HNDO | - |
Pseudomonas sp. |
| 1-hydroxy-2-naphthoic acid dioxygenase | - |
Pseudomonas sp. |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
heat stability for 20 min at 60°C | Pseudomonas sp. |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 70.4 | - |
1-hydroxy-2-naphthoate | - |
Pseudomonas sp. | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Pseudomonas sp. |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | - |
Pseudomonas sp. |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.024 | - |
3-Hydroxy-2-naphthoic acid | competitive inhibition | Pseudomonas sp. | |
| 0.116 | - |
1-hydroxy-2-naphthoic acid | substrate inhibition | Pseudomonas sp. | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | metabolic pathway for degradation of phenanthrene in Pseudomonas sp. strain PPD | Pseudomonas sp. |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5185 | - |
2-Hydroxy-1-naphthoic acid | - |
Pseudomonas sp. | |
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