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Literature summary for 1.13.11.38 extracted from

  • Iwabuchi, T.; Harayama, S.
    Biochemical and molecular characterization of 1-hydroxy-2-naphthoate dioxygenase from Nocardioides sp. KP7 (1998), J. Biol. Chem., 273, 8332-8336.
    View publication on PubMed
Search for more literature for 1.13.11.38

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Nocardioides sp.

Inhibitors

Inhibitors Comment Organism Structure
3-hydroxy-2-naphthoate competitive Nocardioides sp.
additional information no inhibition when the concentration of the substrate 1-hydroxy-2-naphthoate is 0.01 mM and that of the tested compounds is 0.1 or 1 mM: gentisate, 3-hydroxyanthranilate, 2-hydroxy-1-naphthoate, salicylate, m-hydroxybenzoate, p-hydroxybenzoate, and protocatechuate Nocardioides sp.
o-phenanthroline 0.1 mM, reactivation by incubating with FeSO4 and ascorbic acid Nocardioides sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 1-hydroxy-2-naphthoate pH 7.5, 25°C Nocardioides sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ enzyme contains 1.15 mol of Fe(II)/mol of the subunit. Fe2+ is required for the enzyme to exhibit activity Nocardioides sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 6 * 45000, SDS-PAGE Nocardioides sp.
270000
-
 gel filtration Nocardioides sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-hydroxy-2-naphthoate + O2 Nocardioides sp. the enzyme is involved in degradation of phenanthrene (3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate i.e. trans-2'-carboxybenzalpyruvate ?
1-hydroxy-2-naphthoate + O2 Nocardioides sp. KP7 the enzyme is involved in degradation of phenanthrene (3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate i.e. trans-2'-carboxybenzalpyruvate ?

Organism

Organism UniProt Comment Textmining
Nocardioides sp. O24721
-
-
Nocardioides sp. KP7 O24721
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Nocardioides sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:phenanthrene-grown cell
-
 Nocardioides sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.4
-
 enzyme in extract of strain KP7 Nocardioides sp.
180
-
 purified enzyme Nocardioides sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-hydroxy-2-naphthoate + O2
-
 Nocardioides sp. (3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate i.e. trans-2'-carboxybenzalpyruvate ?
1-hydroxy-2-naphthoate + O2 the enzyme is involved in degradation of phenanthrene Nocardioides sp. (3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate i.e. trans-2'-carboxybenzalpyruvate ?
1-hydroxy-2-naphthoate + O2
-
 Nocardioides sp. KP7 (3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate i.e. trans-2'-carboxybenzalpyruvate ?
1-hydroxy-2-naphthoate + O2 the enzyme is involved in degradation of phenanthrene Nocardioides sp. KP7 (3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate i.e. trans-2'-carboxybenzalpyruvate ?
additional information no activity with: gentisate, 3-hydroxyanthranilate, 2-hydroxy-1-naphthoate, 3-hydroxy-2-naphthoate, salicylate, m-hydroxybenzoate, p-hydroxybenzoate, and protocatechuate Nocardioides sp. ?
-
 ?
additional information no activity with: gentisate, 3-hydroxyanthranilate, 2-hydroxy-1-naphthoate, 3-hydroxy-2-naphthoate, salicylate, m-hydroxybenzoate, p-hydroxybenzoate, and protocatechuate Nocardioides sp. KP7 ?
-
 ?

Subunits

Subunits Comment Organism
hexamer 6 * 45000, SDS-PAGE Nocardioides sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Nocardioides sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
114
-
 1-hydroxy-2-naphthoate pH 7.5, 25°C Nocardioides sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Nocardioides sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.022
-
 3-hydroxy-2-naphthoate pH 7.5, 25°C Nocardioides sp.