Application | Comment | Organism |
---|---|---|
medicine | the enzyme is an attractive drug target for the pharmacotherapy of inflammatory and allergic diseases | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
gene alox5, recombinant expression in Escherichia coli strain BL21 | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,5-dimethoxy-3-tridecylbenzene-1,4-diol | - |
Homo sapiens | |
3,4,6-trimethoxy-5-undecylcyclohexa-2,4-dien-1-one | - |
Homo sapiens | |
3-(1,10-dihydroxydecyl)-2,5-dimethoxycyclohexa-2,5-diene-1,4-dione | - |
Homo sapiens | |
3-(1-acetoxy-10-hydroxydecyl)-2,5-dimethoxycyclohexa-2,5-diene-1,4-dione | - |
Homo sapiens | |
3-(10-hydroxydecyl)-2,5-dimethoxycyclohexa-2,5-diene-1,4-dione | - |
Homo sapiens | |
3-(10-hydroxydecyl)-4,5-dimethoxycyclohexa-3,5-diene-1,2-dione | - |
Homo sapiens | |
3-(11-hydroxyundecyl)-2,5-dimethoxycyclohexa-2,5-diene-1,4-dione | - |
Homo sapiens | |
3-(11-hydroxyundecyl)-4,5-dimethoxycyclohexa-3,5-diene-1,2-dione | - |
Homo sapiens | |
3-(8-hydroxyoctyl)-2,5-dimethoxycyclohexa-2,5-diene-1,4-dione | - |
Homo sapiens | |
3-(8-hydroxyoctyl)-4,5-dimethoxycyclohexa-3,5-diene-1,2-dione | - |
Homo sapiens | |
3-dodecyl-4,5-dimethoxybenzene-1,2-diol | - |
Homo sapiens | |
3-dodecyl-4,5-dimethoxycyclohexa-3,5-diene-1,2-dione | - |
Homo sapiens | |
3-tridecyl-4,5-dimethoxybenzene-1,2-diol | shows anti-inflammatory effectiveness | Homo sapiens | |
3-undecyl-4,5-dimethoxybenzene-1,2-diol | - |
Homo sapiens | |
4,5-dimethoxy-3-tridecylcyclohexa-3,5-diene-1,2-dione | shows anti-inflammatory effectiveness | Homo sapiens | |
4,5-dimethoxy-3-undecylcyclohexa-3,5-diene-1,2-dione | - |
Homo sapiens | |
5-methoxy-3-tridecylbenzene-1,2,4-triol | - |
Homo sapiens | |
6-hydroxy-3,4-dimethoxy-5-undecylcyclohexa-2,4-dien-1-one | - |
Homo sapiens | |
embelin | - |
Homo sapiens | |
idebenone | - |
Homo sapiens | |
additional information | high potency of 4,5-dimethoxy-3-alkyl-1,2-benzoquinones on 5-lipoxygenase inhibition is used for systematic structural optimization through accurate structure-based design of the compounds, improvement of the inhibitory potential in vitro and in vivo, overview. Binding patterns of the quinone- and hydroquinone-based 5-LO inhibitors are analyzed by molecular docking, and determination of the optimal alkyl chain pattern of quinones and corresponding hydroquinones | Homo sapiens | |
zileuton | - |
Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required | Homo sapiens | |
Fe2+ | required | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
arachidonate + O2 | Homo sapiens | - |
(5S,6S,7E,9E,11Z,14Z)-5,6-epoxyicosa-7,9,11,14-tetraenoate + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P09917 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain BL21 by ATP affinity chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
neutrophil | stimulated with the Ca2+-mobilizing agent A23187 | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
arachidonate + O2 | - |
Homo sapiens | (5S,6S,7E,9E,11Z,14Z)-5,6-epoxyicosa-7,9,11,14-tetraenoate + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
5-lipoxygenase | - |
Homo sapiens |
5-LO | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00006 | - |
pH 7.4, 37°C, cell-free enzyme extract | Homo sapiens | 3-tridecyl-4,5-dimethoxybenzene-1,2-diol | |
0.00008 | - |
pH 7.4, 37°C, cell-free enzyme extract | Homo sapiens | 4,5-dimethoxy-3-tridecylcyclohexa-3,5-diene-1,2-dione | |
0.00009 | - |
pH 7.4, 37°C, cell-free enzyme extract | Homo sapiens | 4,5-dimethoxy-3-undecylcyclohexa-3,5-diene-1,2-dione | |
0.0001 | - |
pH 7.4, 37°C, cell-free enzyme extract | Homo sapiens | 3-undecyl-4,5-dimethoxybenzene-1,2-diol | |
0.00013 | - |
pH 7.4, 37°C, cell-free enzyme extract | Homo sapiens | 3-dodecyl-4,5-dimethoxycyclohexa-3,5-diene-1,2-dione | |
0.00022 | - |
pH 7.4, 37°C, cell-free enzyme extract | Homo sapiens | 3-(8-hydroxyoctyl)-4,5-dimethoxycyclohexa-3,5-diene-1,2-dione | |
0.00024 | - |
pH 7.4, 37°C, cell-free enzyme extract | Homo sapiens | 3-dodecyl-4,5-dimethoxybenzene-1,2-diol | |
0.00026 | - |
pH 7.4, 37°C, cell-free enzyme extract | Homo sapiens | 2,5-dimethoxy-3-tridecylbenzene-1,4-diol | |
0.00029 | - |
pH 7.4, 37°C, cell-free enzyme extract | Homo sapiens | 5-methoxy-3-tridecylbenzene-1,2,4-triol | |
0.00031 | - |
pH 7.4, 37°C, cell-free enzyme extract | Homo sapiens | 3,4,6-trimethoxy-5-undecylcyclohexa-2,4-dien-1-one | |
0.00049 | - |
pH 7.4, 37°C, cell-free enzyme extract | Homo sapiens | 3-(1-acetoxy-10-hydroxydecyl)-2,5-dimethoxycyclohexa-2,5-diene-1,4-dione | |
0.00092 | - |
pH 7.4, 37°C, cell-free enzyme extract | Homo sapiens | 6-hydroxy-3,4-dimethoxy-5-undecylcyclohexa-2,4-dien-1-one | |
0.00108 | - |
pH 7.4, 37°C, cell-free enzyme extract | Homo sapiens | 3-(10-hydroxydecyl)-4,5-dimethoxycyclohexa-3,5-diene-1,2-dione | |
0.00146 | - |
pH 7.4, 37°C, cell-free enzyme extract | Homo sapiens | 3-(1,10-dihydroxydecyl)-2,5-dimethoxycyclohexa-2,5-diene-1,4-dione | |
0.00151 | - |
pH 7.4, 37°C, cell-free enzyme extract | Homo sapiens | 3-(11-hydroxyundecyl)-2,5-dimethoxycyclohexa-2,5-diene-1,4-dione | |
0.00175 | - |
pH 7.4, 37°C, cell-free enzyme extract | Homo sapiens | 3-(10-hydroxydecyl)-2,5-dimethoxycyclohexa-2,5-diene-1,4-dione | |
0.00184 | - |
pH 7.4, 37°C, cell-free enzyme extract | Homo sapiens | 3-(8-hydroxyoctyl)-2,5-dimethoxycyclohexa-2,5-diene-1,4-dione | |
0.00196 | - |
pH 7.4, 37°C, cell-free enzyme extract | Homo sapiens | 3-(11-hydroxyundecyl)-4,5-dimethoxycyclohexa-3,5-diene-1,2-dione |