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Literature summary for 1.13.11.34 extracted from
- 5-Lipoxygenase from potato tubers. Improved purification and physicochemical characteristics (1987), Biochim. Biophys. Acta, 916, 13-23.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| efficiently stabilized by 20% v/v glycerol | Solanum tuberosum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetonylacetone bisphenylhydrazone | IC50 for reaction with linoleic acid: 0.015 mM, IC50 for reaction with arachidonic acid: 0.0039 mM | Solanum tuberosum |  |
| benzaldehyde phenylhydrazone | IC50 for reaction with linoleic acid: 0.0345 mM, IC50 for reaction with arachidonic acid: 0.0014 mM | Solanum tuberosum | |
| BW755C | CAS: 66000-40-6 | Solanum tuberosum | |
| nordihydroguiaraetic acid | IC50 for reaction with linoleic acid: 0.17 mM, IC50 for reaction with arachidonic acid: 0.0365 mM | Solanum tuberosum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.038 | - |
arachidonic acid | - |
Solanum tuberosum | |
| 0.039 | - |
linoleic acid | - |
Solanum tuberosum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | contains one iron atom per molecule, the native enzyme is in a pseudo-axial high-spin ferric state | Solanum tuberosum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 92000 | - |
x * 92000, SDS-PAGE | Solanum tuberosum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Solanum tuberosum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| major isoenzyme L1 | Solanum tuberosum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| tuber | contains at least 3 isoenzymes | Solanum tuberosum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 147 | - |
- |
Solanum tuberosum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| arachidonate + O2 | i.e. 5,8,11,14-eicosatetraenoic acid | Solanum tuberosum | (6E,8Z,11Z,14Z)-(5S)-hydroperoxyicosa-6,8,11,14-tetraenoate | - |
? | |
| linoleic acid + O2 | - |
Solanum tuberosum | 9-hydroperoxyoctadecadienoic acid | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 92000, SDS-PAGE | Solanum tuberosum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 25 | - |
arachidonic acid | - |
Solanum tuberosum | |
| 138 | - |
linoleic acid | - |
Solanum tuberosum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Solanum tuberosum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 7 | almost inactive at pH 3.5 and at pH 7.0 | Solanum tuberosum |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.0014 | - |
IC50 for reaction with linoleic acid: 0.0345 mM, IC50 for reaction with arachidonic acid: 0.0014 mM | Solanum tuberosum | benzaldehyde phenylhydrazone | |
| 0.0039 | - |
IC50 for reaction with linoleic acid: 0.015 mM, IC50 for reaction with arachidonic acid: 0.0039 mM | Solanum tuberosum | acetonylacetone bisphenylhydrazone | |
| 0.0365 | - |
IC50 for reaction with linoleic acid: 0.17 mM, IC50 for reaction with arachidonic acid: 0.0365 mM | Solanum tuberosum | nordihydroguiaraetic acid | |
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