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Literature summary for 1.13.11.34 extracted from

  • Reddanna, P.; Whelan, J.; Maddipati, K.R.; Reddy, C.C.
    Purification of arachidonate 5-lipoxygenase from potato tubers (1990), Methods Enzymol., 187, 268-277.
    View publication on PubMed
Search for more literature for 1.13.11.34

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
 arachidonic acid
-
 Solanum tuberosum
0.033
-
 arachidonic acid
-
 Solanum tuberosum
0.05
-
 bishomo-gamma-linolenic acid
-
 Solanum tuberosum
0.2
-
 linoleic acid
-
 Solanum tuberosum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 x * 35000, enzyme form L2, SDS-PAGE Solanum tuberosum
85000
-
 enzyme form L1 and L2, gel filtration Solanum tuberosum

Organism

Organism UniProt Comment Textmining
Solanum tuberosum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Solanum tuberosum

Source Tissue

Source Tissue Comment Organism Textmining
tuber
-
 Solanum tuberosum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.7
-
 enzyme form L2 Solanum tuberosum
13.7
-
 enzyme form L1 Solanum tuberosum

Storage Stability

Storage Stability Organism
-20°C, 40 mM potassium phosphate buffer, pH 6.3, 2 M (NH4)2SO4, enzyme form L1 is stable for months Solanum tuberosum
4°C, the L1 form is gradually transformed into the L2 form with a significant reduction in specific activity, the L2 form loses all of its activity over a few days and more rapid in absence of (NH4)2SO4 Solanum tuberosum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arachidonate + O2 i.e. 5,8,11,14-eicosatetraenoic acid Solanum tuberosum (6E,8Z,11Z,14Z)-(5S)-hydroperoxyicosa-6,8,11,14-tetraenoate 5(S)-hydroperoxyeicosatetraenoic acid is the most abundant primary oxygenation product, representing 60% of the total hydroperoxyeicosatetraenoic acids, followed by 8(S)-hydroperoxyeicosatetraenoic acid, 9-hydroperoxyeicosatetraenoic acid, 11(S)-hydroperoxyeicosatetraenoic acid, 12-hydroperoxyeicosatetraenoic acid, and 15-hydroperoxyeicosatetraenoic acid in decreasing order of abundance, all possible diH(P)ETEs are detected ?
bishomo-gamma-linolenic acid + O2
-
 Solanum tuberosum ?
-
 ?

Subunits

Subunits Comment Organism
? x * 35000, enzyme form L2, SDS-PAGE Solanum tuberosum
? x * 85000, enzyme form L2, SDS-PAGE Solanum tuberosum
More the L1 form is gradually transformed into the L2 form with a significant reduction in specific activity Solanum tuberosum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 pH of the reaction has profound influence on the product profile Solanum tuberosum