Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | positional specificity of wild-type 15-LOX-2 does hardly depend on the pH of the reaction buffer. The pH sensitivity can be induced by introduction of a His at the active site | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
ligated into the pQE-9 plasmid and expressed as N-terminal His-tag fusion protein in Escherichia coli (XL-1 Blue) | Mus musculus |
ligated into the pQE-9 plasmid and expressed as N-terminal His-tag fusion protein in Escherichia coli (XL-1 Blue) | Oryctolagus cuniculus |
LOX isoforms ligated into the pQE-9 plasmid and expressed as N-terminal His-tag fusion protein in Escherichia coli (XL-1 Blue) | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D602Y | 15-LOX-2 mutant shows a similar pH-profile as the wild-type enzyme | Homo sapiens |
D602Y/V603H | a strong shift of the pH profile of 15-LOX-2 mutant towards more acidic values | Homo sapiens |
V603H | a strong shift of the pH profile of 15-LOX-2 mutant towards more acidic values. 15-H(p)ETE is the major oxygenation product at pH 8 | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | physico-chemical state of the substrate and the complex equilibrium between fatty acid monomers, acid soaps and micelles may impact the reaction specificity of LOX-isoforms | Homo sapiens | |
additional information | physico-chemical state of the substrate and the complex equilibrium between fatty acid monomers, acid soaps and micelles may impact the reaction specificity of LOX-isoforms | Mus musculus | |
additional information | pH alterations in the near physiological range impact the iron content of LOX and thus the catalytic activity | Oryctolagus cuniculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | - |
Oryctolagus cuniculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Mus musculus | - |
- |
- |
Oryctolagus cuniculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
on Ni column | Mus musculus |
on Ni column | Homo sapiens |
on Ni column | Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
skin | - |
Mus musculus | - |
Storage Stability | Organism |
---|---|
-80°C, 10% glycerol | Mus musculus |
-80°C, 10% glycerol | Homo sapiens |
-80°C, 10% glycerol | Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
arachidonate + O2 | - |
Mus musculus | (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoate | - |
? | |
arachidonate + O2 | - |
Oryctolagus cuniculus | (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoate | 15-H(p)ETE is the major reaction product independent of the pH | ? | |
arachidonate + O2 | - |
Homo sapiens | (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoate | 15-H(p)ETE is the major reaction product of 15-LOX-2 and 12/15-LOX independent of the pH | ? | |
additional information | 15-LOX-2 does not oxygenate (5Z,8Z,11Z,14Z)-nonadeca-5,8,11,14-tetraene-1,19-dioic acid at various pH | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
12/15-LOX | - |
Mus musculus |
12/15-LOX | - |
Homo sapiens |
12/15-LOX | - |
Oryctolagus cuniculus |
15-lipoxygenase | - |
Mus musculus |
15-lipoxygenase-2 | - |
Homo sapiens |
15-LOX-1 | - |
Homo sapiens |
15-LOX-2 | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | pH-optimum of 15-LOX-2 for arachidonic acid oxygenation | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9.5 | pH 6 about 50% of maximal activity, pH 9.5 about 60% of maximal activity | Homo sapiens |