Cloned (Comment) | Organism |
---|---|
expression of isozyme 15-hLO-1 as His-tagged enzyme, and of untagged isozyme 15-hLO-2 | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0025 | - |
arachidonic acid | 13,13-dideuterated arachidonic acid, pH 7.5, 25°C, recombinant isozyme 15-hLO-1 | Homo sapiens | |
0.0028 | - |
arachidonic acid | 10,10,13,13-tetradeuterated arachidonic acid, pH 7.5, 25°C, recombinant isozyme 15-hLO-1 | Homo sapiens | |
0.0034 | - |
arachidonic acid | 10,10-dideuterated arachidonic acid, pH 7.5, 25°C, recombinant isozyme 15-hLO-1 | Homo sapiens | |
0.0037 | - |
arachidonic acid | unlabeled substrate, pH 7.5, 25°C, recombinant isozyme 15-hLO-1 | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required for the catalytic cycle of fatty acid oxidation by lipoxygenase, overview | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
arachidonate + O2 | Homo sapiens | arachidonic acid metabolization by lipoxygenases, overview | (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyeicosa-5,8,11,13-tetraenoate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
two human isozymes: platelet 12-hLO, reticulocyte 15-hLO-1, and epithelial 15-hLO-2 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged isozyme 15-hLO-1 by nickel affinity chromatography, recombinant untagged isozyme 15-hLO-2 by ion exchange chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
epithelium | isozyme 15-hLO-2 | Homo sapiens | - |
reticulocyte | isozyme 15-hLO-1 | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
arachidonate + O2 | arachidonic acid metabolization by lipoxygenases, overview | Homo sapiens | (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyeicosa-5,8,11,13-tetraenoate | - |
? | |
arachidonate + O2 | regioselective oxidation is achieved through control over the position of hydrogen atom abstraction by the geometry and size of the enzyme active site and through control by the protein over the interaction of molecular oxygen with the generated delocalized substrate radical, analysis of catalytic mechanisms of lipoxygenases using 10,10-dideuterated arachidonic acid, 13,13-dideuterated arachidonic acid, and 0,10,13,13-d4-AA, overview | Homo sapiens | (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyeicosa-5,8,11,13-tetraenoate | isozymes 15-hLO-1 and 15-hLO-2 also form small amounts of 11-HPETE, 8-HPETE, and 12-HPETE, the rate of 8/12-HPETE production by isozyme 15-hLO-1 with 13,13-dideuterated arachidonic acid as substrate is exeptionally high with 75% of overall production, overview | ? | |
additional information | product distributions of lipoxygenases under various conditions, overview | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
15-lipoxygenase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
arachidonic acid | 10,10,13,13-tetradeuterated arachidonic acid, pH 7.5, 25°C, recombinant isozyme 15-hLO-1 | Homo sapiens | |
2.49 | - |
arachidonic acid | 13,13-dideuterated arachidonic acid, pH 7.5, 25°C, recombinant isozyme 15-hLO-1 | Homo sapiens | |
9.26 | - |
arachidonic acid | 10,10-dideuterated arachidonic acid, pH 7.5, 25°C, recombinant isozyme 15-hLO-1 | Homo sapiens | |
9.93 | - |
arachidonic acid | unlabeled substrate, pH 7.5, 25°C, recombinant isozyme 15-hLO-1 | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |