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Literature summary for 1.13.11.33 extracted from

  • Coffa, G.; Brash, A.R.
    A single active site residue directs oxygenation stereospecificity in lipoxygenases: Stereocontrol is linked to the position of oxygenation (2004), Proc. Natl. Acad. Sci. USA, 101, 15579-15584.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.11.33

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
A416T inactive mutant protein Homo sapiens
A416V inactive mutant protein Homo sapiens
A417G the mutant enzyme converts arachidonic acid to (11R)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoic acid and (15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoic acid in a 1.5:1 ratio. The wild.type enzyme converts arachidonic acid exlusively (15S)-Hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoic acid. Turnover number for arachidonic acid is 4.6fold lower than that of the wild-type enzyme, no significant change in Km-value Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arachidonic acid + O2
-
 Homo sapiens (15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoic acid the mutant enzyme A416G converts arachidonic acid to (11R)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoic acid and (15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoic acid in a 1.5:1 ratio ?

Synonyms

Synonyms Comment Organism
15-LOX-2
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3
-
 arachidonate mutant enzyme A416G Homo sapiens
14
-
 arachidonate wild-type enzyme Homo sapiens