Literature summary for 1.13.11.31 extracted from

Flatman, S.; Hurst, J.S.; McDonald-Gibson, R.G.; Jonas, G.E.G.; Slater, T.F.
Biochemical studies on a 12-lipoxygenase in human uterine cervix (1986), Biochim. Biophys. Acta, 883, 7-14.

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Activating Compound

Activating Compound	Comment	Organism	Structure
ATP	2 mM ATP + 1 mM CaCl2 stimulate approximately 2fold	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
caffeic acid	0.01 mM, 20-30% inhibition	Homo sapiens
eicosatetraynoic acid	0.01 mM, 90-95% inhibition	Homo sapiens
esculetin	0.01 mM, 90-95% inhibition	Homo sapiens
iodoacetate	1 mM, 5% inhibition	Homo sapiens
Lubrol	0.2%, 60% inhibition	Homo sapiens
NEM	1 mM, 15% inhibition	Homo sapiens
nordihydroguaiaretic acid	0.01 mM, 90-95% inhibition	Homo sapiens
p-hydroxymercuribenzoate	1 mM, 75% inhibition	Homo sapiens
Renex	-	Homo sapiens
Triton X-100	-	Homo sapiens
Tween 20	-	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.015	-	arachidonic acid	-	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	2 mM ATP + 1 mM CaCl2 stimulate approximately 2fold	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
uterine cervix	-	Homo sapiens	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Homo sapiens

Storage Stability

Storage Stability	Organism
-20°C, remains stable for 6 weeks	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
arachidonate + O2	i.e. 5Z,8Z,11Z,14Z-eicosatetraenoic acid	Homo sapiens	(5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyicosa-5,8,10,14-tetraenoate	-	?

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
4	-	90% loss of activity after 4 h	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	-	Homo sapiens

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Release 2023.1 (January 2023)