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Literature summary for 1.13.11.3 extracted from

  • Da Silva, A.; Jacques, R.; Andreazza, R.; Bento, F.; Camargo, F.
    The effects of trace elements, cations, and environmental conditions on protocatechuate 3,4-dioxygenase activity (2013), Sci. Agric. (Piracicaba, Braz.), 70, 68-73.
No PubMed abstract available
Search for more literature for 1.13.11.3

Cloned(Commentary)

Cloned (Comment) Organism
phylogenetic tree Leifsonia sp.

Protein Variants

Protein Variants Comment Organism
additional information immobilization of the enzyme, the immobilized extract exhibited higher enzyme activity than the cell-free extract in the presence of trace elements and cations Leifsonia sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ increases enzyme activity for both cell-free and immobilized extracts by 8% and 44%, respectively Leifsonia sp.
Fe3+ increases enzyme activity for both cell-free and immobilized extracts by 16% and 99%, respectively Leifsonia sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3,4-dihydroxybenzoate + O2 Leifsonia sp.
-
 3-carboxy-cis,cis-muconate
-
 ?

Organism

Organism UniProt Comment Textmining
Leifsonia sp.
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:phenanthrene-grown cell
-
 Leifsonia sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,4-dihydroxybenzoate + O2
-
 Leifsonia sp. 3-carboxy-cis,cis-muconate
-
 ?

Synonyms

Synonyms Comment Organism
P3,4O enzyme
-
 Leifsonia sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
 optimum for the cell-free enzyme extract Leifsonia sp.
55
-
 optimum for the immobilized enzyme extract Leifsonia sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Leifsonia sp.

pH Range

pH Minimum pH Maximum Comment Organism
4 9 activity range, profile overview Leifsonia sp.
6.5 8 80% of maximal activity within this range Leifsonia sp.