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Literature summary for 1.13.11.3 extracted from

  • Bubinas, A.; Giedraityte, G.; Kalediene, L.; Nivinskiene, O.; Butkiene, R.
    Degradation of naphthalene by thermophilic bacteria via a pathway, through protocatechuic acid (2008), Cent. Eur. J. Biol., 3, 61-68.
No PubMed abstract available
Search for more literature for 1.13.11.3

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Geobacillus sp. enzyme involved in last ring fission in naphtalene degradation, intermediates confirm proceeding through protocatechuic acid via ortho-claevage pathway ?
-
 ?
protocatechuic acid + O2 Geobacillus sp.
-
 3-carboxy-cis,cis-muconic acid
-
 ?

Organism

Organism UniProt Comment Textmining
Geobacillus sp.
-
 thermophilic isolate G27 from high-temperature oilfield in Lithuania
-

Purification (Commentary)

Purification (Comment) Organism
cells harvested by centrifugation, washed twice with 50 mM sodium phosphate buffer, pH 7.0 containing 10% glycerol, disruption with solicitor, centrifuged, supernatant stored at -20°C Geobacillus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
350
-
 activity at the 10th h of the exponential growth phase Geobacillus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme involved in last ring fission in naphtalene degradation, intermediates confirm proceeding through protocatechuic acid via ortho-claevage pathway Geobacillus sp. ?
-
 ?
protocatechuic acid + O2
-
 Geobacillus sp. 3-carboxy-cis,cis-muconic acid
-
 ?

Synonyms

Synonyms Comment Organism
3,4-PCDase
-
 Geobacillus sp.
protocatchetuate 3,4-dioxygenase
-
 Geobacillus sp.