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Literature summary for 1.13.11.3 extracted from

  • Chen, Y.P.; Dilworth, M.J.; Glenn, A.R.
    Aromatic metabolism in Rhizobium trifolii: protocatechuate 3,4-dioxygenase (1984), Arch. Microbiol., 138, 187-190.
No PubMed abstract available
Search for more literature for 1.13.11.3

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 0.005 mM, approx. 50% inhibition after 30 min, complete inhibition after 60 min, complete restoration of inactivated enzyme by addition of excess ferric EDTA complex Rhizobium leguminosarum
Al3+ EDTA salt, 0.01 mM, complete inhibition Rhizobium leguminosarum
Ni2+ EDTA salt, 0.01 mM, 72% inhibition Rhizobium leguminosarum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0175
-
 protocatechuate
-
 Rhizobium leguminosarum

Metals/Ions

Metals/Ions Comment Organism Structure
Fe 1.3-1.5 g-atom Fe3+ per mol of enzyme Rhizobium leguminosarum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26500
-
 alpha,beta, 4 * 29000 + 4 * 26500, SDS-PAGE Rhizobium leguminosarum
29000
-
 alpha,beta, 4 * 29000 + 4 * 26500, SDS-PAGE Rhizobium leguminosarum
220000
-
 gel filtration Rhizobium leguminosarum

Organism

Organism UniProt Comment Textmining
Rhizobium leguminosarum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, Sephacryl S 300, DEAE-Sephadex A50 Rhizobium leguminosarum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,4-dihydroxybenzoate + O2 specific for protocatechuate Rhizobium leguminosarum 3-carboxy-cis,cis-muconate
-
 ?

Subunits

Subunits Comment Organism
octamer alpha,beta, 4 * 29000 + 4 * 26500, SDS-PAGE Rhizobium leguminosarum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.2
-
-
 Rhizobium leguminosarum