Literature summary for 1.13.11.27 extracted from

Rocaboy-Faquet, E.; Noguer, T.; Romdhane, S.; Bertrand, C.; Dayan, F.E.; Barthelmebs, L.
Novel bacterial bioassay for a high-throughput screening of 4-hydroxyphenylpyruvate dioxygenase inhibitors (2014), Appl. Microbiol. Biotechnol., 98, 7243-7252 .

Search for more literature for 1.13.11.27

Application

Application	Comment	Organism
agriculture	plant 4-hydroxyphenylpyruvate dioxygenase (HPPD) is the molecular target of a range of commercial synthetic beta-triketone herbicides Their mode of action is based on an irreversible inhibition of HPPD	Arabidopsis thaliana
drug development	plant 4-hydroxyphenylpyruvate dioxygenase (HPPD) is the molecular target for development of specific inhibitors	Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment)	Organism
gene HPD, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha, determination of protein expression patterns	Arabidopsis thaliana

Inhibitors

Inhibitors	Comment	Organism	Structure
leptospermone	-	Arabidopsis thaliana
mesotrione	-	Arabidopsis thaliana
additional information	high-throughput screening of 4-hydroxyphenylpyruvate dioxygenase inhibitors	Arabidopsis thaliana
sulcotrione	-	Arabidopsis thaliana
tembotrione	-	Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
4-hydroxyphenylpyruvate + O2	Arabidopsis thaliana	-	homogentisate + CO2	-	?

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	P93836	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography	Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-hydroxyphenylpyruvate + O2	-	Arabidopsis thaliana	homogentisate + CO2	-	?

Subunits

Subunits	Comment	Organism
?	x * 48000, recombinant His-tagged enzyme, SDS-PAGE	Arabidopsis thaliana

Synonyms

Synonyms	Comment	Organism
HPD	-	Arabidopsis thaliana
HPPD	-	Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Arabidopsis thaliana

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.6	-	assay at	Arabidopsis thaliana

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)