Literature summary for 1.13.11.27 extracted from

Lederer, B.; Boeger, P.
Recombinant p-hydroxyphenylpyruvate dioxygenase of high activity (2005), Z. Naturforsch. C, 60, 549-556.

Search for more literature for 1.13.11.27

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	regarding expression and acitvity, rich or complex media (surplus of yeast extract, addition of glycerol and buffering of pH) are superior to media with less complex composition. Supplementation of media with FeSO4 or tyrosine does not substantially upgrade expression and activity levels.	Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Eschrichia coli stains DH5alpha, M15(pRep4) or SG13009	Arabidopsis thaliana

General Stability

General Stability	Organism
regeneration of time-dependent inactivated 4-hydroxyphenylpyruvate dioxygenase by incubation with ascorbic acid and/or Fe2+ salts is not successful	Arabidopsis thaliana

Inhibitors

Inhibitors	Comment	Organism	Structure
sulcotrione	for protein expressed in Escherichia coli DH5alpha (inhibition assayed without preincubation): 0 micoM sulcotrione = 100% activity, 0.5 microM sulcotrione = 58% activity left, 1.0 microM sulcotrione = 21.3% activity left, 1.5 microM sulcotrione = 2.8% activity left. Preincubation of the enzyme expressed in Escherichia coli SG13009 with sulcotrione for 15 min, significantly enhances the inhibition from 40% (1 microM sulcotrione/no preincubation) to 10% (1 microM sulcotrione/15 min preincubation).	Arabidopsis thaliana

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	P93836	sequence of the cloned 4-hydroxyphenylpyruvate dioxygenase coincides with published 4-hydroxyphenylpyruvate dioxygenase-sequences from Arabidopsis thaliana to a nearly perfect identity match except for minor differences in the C-terminal parts of the cDNA.	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	drought-stressed Arabidopsis thaliana leaf RNA	Arabidopsis thaliana	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	activity of 0.0033 micromol/min/mg for 2YT-grown Escherichia coli DH5alpha extract and 0.0018 micromol/min/mg for LB-grown Escherichia coli DH5alpha	Arabidopsis thaliana
0.004	-	protein expressed in Escherichia coli DH5alpha	Arabidopsis thaliana
0.11	0.2	protein expressed in Escherichia coli SG13009	Arabidopsis thaliana
0.17	0.26	protein expressed in Escherichia coli M15(pRep4)	Arabidopsis thaliana

Storage Stability

Storage Stability	Organism
storage at room temperature results in residual enzyme activities of ca. 60-80% after 16 h or 40-50% after 30 h.	Arabidopsis thaliana

Synonyms

Synonyms	Comment	Organism
HPPD	-	Arabidopsis thaliana

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
-20	-	enzyme activity is stable for a few weeks.	Arabidopsis thaliana

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Release 2023.1 (January 2023)