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Literature summary for 1.13.11.27 extracted from

  • Lindstedt, S.; Odelhoeg, B.; Rundgren, M.
    Purification and some properties of 4-hydroxyphenylpyruvate dioxygenase from Pseudomonas sp. P. J. 874 (1977), Biochemistry, 16, 3369-3377.
    View publication on PubMed
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Activating Compound

Activating Compound Comment Organism Structure
2,6-dichlorophenol indophenol competitive activator Pseudomonas sp.
ascorbate an optimized combination of ascorbate, 50 mM, catalase, 2.4 g/l, and iron, 0.05 mM, activates more than a combination of 2,6-dichlorophenolindophenol, glutathione and catalase Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
2,2'-bipyridyl 0.32 mM, 50% inhibition Pseudomonas sp.
4-hydroxyphenylpyruvate above 0.2 mM Pseudomonas sp.
8-Hydroxyquinoline-5-sulfonic acid 560 nM, 50% inhibition Pseudomonas sp.
Bathocuproine 3 mM, 50% inhibition Pseudomonas sp.
bathophenanthroline 0.012 mM, 50% inhibition Pseudomonas sp.
citrate
-
 Pseudomonas sp.
Cupferron 0.0071 mM, 50% inhibition Pseudomonas sp.
diethyldithiocarbamate 0.0021 mM, 50% inhibition Pseudomonas sp.
EDTA
-
 Pseudomonas sp.
H2O2 10 mM, 30% inhibition Pseudomonas sp.
Tiron 0.4 mM, 50% inhibition Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.52
-
 phenylpyruvate
-
 Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
copper variable amounts of copper: 0.2-0.5 mol per mol of enzyme Pseudomonas sp.
Fe2+ essential role for enzyme bound zinc Pseudomonas sp.
Iron variable amounts of iron: 0.6-1.3 mol per mol of enzyme Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 4 * 36000, SDS-PAGE Pseudomonas sp.
150000
-
 high-speed equilibrium sedimentation Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. P.J. 874
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.92
-
-
 Pseudomonas sp.

Storage Stability

Storage Stability Organism
-60°C, 0.2 M NaCl solution buffered with 10 mM potassium phosphate, pH 6.7, stable for several months Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-hydroxyphenylpyruvate + O2
-
 Pseudomonas sp. homogentisate + CO2
-
 ?
4-hydroxyphenylpyruvate + O2
-
 Pseudomonas sp. P.J. 874 homogentisate + CO2
-
 ?
phenylpyruvate + O2
-
 Pseudomonas sp. 2-hydroxyphenylacetate + CO2
-
 ?
phenylpyruvate + O2
-
 Pseudomonas sp. P.J. 874 2-hydroxyphenylacetate + CO2
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 36000, SDS-PAGE Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7.5
-
 Pseudomonas sp.