Literature summary for 1.13.11.27 extracted from

Lindstedt, S.; Rundgren, M.
Blue color, metal content, and substrate binding in 4-hydroxyphenylpyruvate dioxygenase from Pseudomonas sp. strain P. J. 874 (1982), J. Biol. Chem., 257, 11922-11931.

Search for more literature for 1.13.11.27

Inhibitors

Inhibitors	Comment	Organism
1,2-dihydroxybenzene	0.019 mM, 50% inhibition, uncompetitive	Pseudomonas sp.
1,4-benzoquinone	0.25 mM, 50% inhibition, competitive	Pseudomonas sp.
1,4-dihydroxybenzene	4.7 mM, 50% inhibition	Pseudomonas sp.
2,4-Dihydroxybenzoate	4.2 mM, 50% inhibition, noncompetitive	Pseudomonas sp.
2,5-Dihydroxybenzoate	2.6 mM, 50% inhibition, noncompetitive	Pseudomonas sp.
2,5-dihydroxyphenylacetate	50% inhibition above 10 mM, noncompetitive	Pseudomonas sp.
2,5-dihydroxyphenylacetic acid lactone	50% inhibition above 10 mM, competitive	Pseudomonas sp.
2-hydroxyphenylacetate	10 mM, 50% inhibition, noncompetitive	Pseudomonas sp.
3,4-dihydroxybenzoate	0.24 mM, 50% inhibition, noncompetitive	Pseudomonas sp.
3,4-Dihydroxyphenylacetate	0.75 mM, 50% inhibition, noncompetitive	Pseudomonas sp.
3,5-diiodo-4-hydroxyphenylpyruvate	0.25 mM, 50% inhibition, competitive	Pseudomonas sp.
3-(2-hydroxyphenyl)propionate	50% inhibition above 10 mM, competitive	Pseudomonas sp.
3-methoxy-4-hydroxyphenylacetate	0.25 mM, 50% inhibition, competitive	Pseudomonas sp.
4-hydroxybenzaldehyde	50% inhibition above 10 mM, noncompetitive	Pseudomonas sp.
4-hydroxyphenylpyruvate	substrate inhibition	Pseudomonas sp.
Acetopyruvate	0.33 mM, 50% inhibition, competitive	Pseudomonas sp.
bathophenanthroline	-	Pseudomonas sp.
diethyl dicarbonate	5 mM, 50% inhibition	Pseudomonas sp.
DL-3,4-dihydroxyphenylalanine	0.32 mM, 50% inhibition, noncompetitive	Pseudomonas sp.
DL-4-hydroxyphenyllactate	50% inhibition above 10 mM, competitive	Pseudomonas sp.
DL-Epinephrine	0.17 mM, 50% inhibition, noncompetitive	Pseudomonas sp.
ethanol	5% v/v, 50% inhibition, complete loss of activity at 20% v/v	Pseudomonas sp.
H2O2	0.01 mM-1 mM, 5-15% inhibition	Pseudomonas sp.
L-Phe	10 mM, 50% inhibition, competitive	Pseudomonas sp.
oxaloacetate	10 mM, 50% inhibition, noncompetitive	Pseudomonas sp.
phenylglyoxalate	50% inhibition above 10 mM, competitive	Pseudomonas sp.
phenylpyruvate	0.53 mM, 50% inhibition, competitive	Pseudomonas sp.
pyridoxal 5'-phosphate	2 mM, 50% inhibition	Pseudomonas sp.
pyruvate	50% inhibition above 10 mM, competitive	Pseudomonas sp.
trans-4-hydroxycinnamate	50% inhibition above 10 mM, competitive	Pseudomonas sp.

Metals/Ions

Metals/Ions	Comment	Organism
Fe2+	the enol tautomer of 4-hydroxyphenylpyruvate appears to form a 2:1 complex with enzyme-bound Fe2+, the tautomerizable keto group with a double bond in 3-4 position favors productive substrate binding to Fe2+	Pseudomonas sp.
Iron	0.95 mol of iron per mol of enzyme	Pseudomonas sp.
Zinc	0.68 mol of zinc per mol of enzyme	Pseudomonas sp.

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas sp.	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-hydroxyphenylpyruvate + O2	-	Pseudomonas sp.	homogentisate + CO2	-	?

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
37	-	pH 5.5-8, 15 min, stable	Pseudomonas sp.

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	-	Pseudomonas sp.

pH Range

pH Minimum	pH Maximum	Comment	Organism
6	9.5	pH 6.0: about 55% of maximal activity, pH 9.5: about 50% of maximal activity	Pseudomonas sp.

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
5.5	8	37°C, 15 min, stable	Pseudomonas sp.

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
additional information	-	additional information	-	Pseudomonas sp.