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Literature summary for 1.13.11.27 extracted from

  • Lindstedt, S.; Odelhoeg, B.
    4-Hydroxyphenylpyruvate dioxygenase from Pseudomonas (1987), Methods Enzymol., 142, 143-148.
    View publication on PubMed
Search for more literature for 1.13.11.27

Inhibitors

Inhibitors Comment Organism Structure
4-hydroxyphenylpyruvate above 0.2 mM; substrate inhibition Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
 4-hydroxyphenylpyruvate
-
 Pseudomonas sp.
0.5
-
 phenylpyruvate
-
 Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ essential role for enzyme-bound Fe2+ in catalytic process Pseudomonas sp.
Fe3+ iron in the Fe3+ form must be reduced for enzyme activity Pseudomonas sp.
Iron 0.95 mol of iron per mol of enzyme Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 4 * 36000, SDS-PAGE Pseudomonas sp.
150000
-
 high-speed equilibrium sedimentation Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. P.J. 874
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1
-
-
 Pseudomonas sp.

Storage Stability

Storage Stability Organism
-60°C, stable for several months Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-hydroxyphenylpyruvate + O2
-
 Pseudomonas sp. homogentisate + CO2
-
 ?
4-hydroxyphenylpyruvate + O2
-
 Pseudomonas sp. P.J. 874 homogentisate + CO2
-
 ?
phenylpyruvate + O2
-
 Pseudomonas sp. 2-hydroxyphenylacetate + CO2
-
 ?
phenylpyruvate + O2
-
 Pseudomonas sp. P.J. 874 2-hydroxyphenylacetate + CO2
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 36000, SDS-PAGE Pseudomonas sp.