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Literature summary for 1.13.11.26 extracted from
- Separation of tryptophan pyrrolooxygenase into three molecular forms. A study of their substrate specificities using tryptophyl-containing peptides and proteins (1986), Eur. J. Biochem., 156, 317-325.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 2 mM, total activity loss | Triticum aestivum |  |
| alpha,alpha'-dipyridyl | - |
Triticum aestivum | |
| dithioerythritol | 2 mM, total activity loss | Triticum aestivum | |
| dithiothreitol | 2 mM, total activity loss | Triticum aestivum | |
| EDTA | - |
Triticum aestivum | |
| o-phenanthroline | - |
Triticum aestivum | |
| p-hydroxymercurisulfonate | 10 mM, activity loss of 60% | Triticum aestivum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25000 | - |
molecular form A, SDS-PAGE | Triticum aestivum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-tryptophan + O2 | Triticum aestivum | - |
formylkynurenine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Triticum aestivum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| three molecular forms: A,B,C | Triticum aestivum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| germ | - |
Triticum aestivum | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, forms A and C stable for at least two weeks, form B activity loss after three days | Triticum aestivum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-tryptophan + O2 | - |
Triticum aestivum | formylkynurenine | - |
? | |
| DL-5-fluorotryptophan + O2 | - |
Triticum aestivum | 5-fluoroformylkynurenine | - |
? | |
| DL-5-methyltryptophan + O2 | - |
Triticum aestivum | 5-methyl-N-formylkynurenine | - |
? | |
| DL-6-fluorotryptophan + O2 | - |
Triticum aestivum | 4-fluoro-N-formylkynurenine | - |
? | |
| DL-6-methyltryptophan + O2 | - |
Triticum aestivum | 4-methyl-N-formylkynurenine | - |
? | |
| DL-7-methyltryptophan + O2 | - |
Triticum aestivum | 3-methyl-N-formylkynurenine | - |
? | |
| DL-tryptophan + O2 | - |
Triticum aestivum | formylkynurenine | - |
? | |
| DL-tryptophan methyl ester + O2 | - |
Triticum aestivum | formylkynurenine methyl ester | - |
? | |
| L-5-hydroxytryptophan + O2 | very poor substrate activity with molecular form A | Triticum aestivum | 5-hydroxy-N-formylkynurenine | - |
? | |
| L-tryptophan + O2 | - |
Triticum aestivum | formylkynurenine | - |
? | |
| N-acetyl-5-methyltryptophan + O2 | - |
Triticum aestivum | 5-methyl-Nalpha-acetyl-Nomega-formylkynurenine | - |
? | |
| N-acetyl-DL-tryptophan + O2 | - |
Triticum aestivum | N-acetylformylkynurenine | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
form C with chymotrypsin as substrate | Triticum aestivum |
| 37 | - |
form B, form C with tryptophan as substrate | Triticum aestivum |
| 37 | 45 | form A, tryptophan or gamma-chymotrypsin as substrates | Triticum aestivum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.6 | - |
in Tris-HCl buffer | Triticum aestivum |
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