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Literature summary for 1.13.11.24 extracted from
- Excess production of Bacillus subtilis quercetin 2,3-dioxygenase affects cell viability in the presence of quercetin (2010), Biosci. Biotechnol. Biochem., 74, 1030-1038.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Bacillus subtilis |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | required for activity | Bacillus subtilis |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
about 40000 Da, SDS-PAGE | Bacillus subtilis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| quercetin + O2 | Bacillus subtilis | - |
2-protocatechoylphloroglucinolcarboxylate + CO | - |
? | |
| quercetin + O2 | Bacillus subtilis 168 | - |
2-protocatechoylphloroglucinolcarboxylate + CO | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
| Bacillus subtilis 168 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial purification by ammonium sulfate precipitation and DEAE Toyopearl column chromatography | Bacillus subtilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0012 | - |
native enzyme, using quercetin as substrate, at 25°C, pH 7.5 | Bacillus subtilis |
| 0.0276 | - |
recombinant enzyme, using fisetin as substrate, at 25°C, pH 7.5 | Bacillus subtilis |
| 1.87 | - |
recombinant enzyme, using tamarixetin as substrate, at 25°C, pH 7.5 | Bacillus subtilis |
| 2.27 | - |
recombinant enzyme, using quercetin as substrate, at 25°C, pH 7.5 | Bacillus subtilis |
| 2.49 | - |
recombinant enzyme, using galangin as substrate, at 25°C, pH 7.5 | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| fisetin + O2 | 1.22% activity compared to quercetin | Bacillus subtilis | 2-[[(3,4-dihydroxyphenyl)carbonyl]oxy]-4-hydroxybenzoate + CO | - |
? | |
| fisetin + O2 | 1.22% activity compared to quercetin | Bacillus subtilis 168 | 2-[[(3,4-dihydroxyphenyl)carbonyl]oxy]-4-hydroxybenzoate + CO | - |
? | |
| galangin + O2 | 110% activity compared to quercetin | Bacillus subtilis | 2,4-dihydroxy-6-[(phenylcarbonyl)oxy]benzoate + CO | - |
? | |
| galangin + O2 | 110% activity compared to quercetin | Bacillus subtilis 168 | 2,4-dihydroxy-6-[(phenylcarbonyl)oxy]benzoate + CO | - |
? | |
| quercetin + O2 | - |
Bacillus subtilis | 2-protocatechoylphloroglucinolcarboxylate + CO | - |
? | |
| quercetin + O2 | 100% activity | Bacillus subtilis | 2-protocatechoylphloroglucinolcarboxylate + CO | - |
? | |
| quercetin + O2 | - |
Bacillus subtilis 168 | 2-protocatechoylphloroglucinolcarboxylate + CO | - |
? | |
| quercetin + O2 | 100% activity | Bacillus subtilis 168 | 2-protocatechoylphloroglucinolcarboxylate + CO | - |
? | |
| tamarixetin + O2 | 82.4% activity compared to quercetin | Bacillus subtilis | 2,4-dihydroxy-6-[[(3-hydroxy-4-methoxyphenyl)carbonyl]oxy]benzoate + CO | - |
? | |
| tamarixetin + O2 | 82.4% activity compared to quercetin | Bacillus subtilis 168 | 2,4-dihydroxy-6-[[(3-hydroxy-4-methoxyphenyl)carbonyl]oxy]benzoate + CO | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| QdoI | - |
Bacillus subtilis |
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