Literature summary for 1.13.11.24 extracted from

Tranchimand, S.; Ertel, G.; Gaydou, V.; Gaudin, C.; Tron, T.; Iacazio, G.
Biochemical and molecular characterization of a quercetinase from Penicillium olsonii (2008), Biochimie, 90, 781-789.

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Cloned(Commentary)

Cloned (Comment)	Organism
-	Penicillium olsonii

Inhibitors

Inhibitors	Comment	Organism	Structure
1H-2-benzyl-3-hydroxy-4-oxoquinolin	competitive	Penicillium olsonii
diethyldithiocarbamate	100 nM, complete inhibition	Penicillium olsonii

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.013	-	kaempferol	pH 6, 25°C	Penicillium olsonii
0.019	-	quercetin	pH 6, 25°C	Penicillium olsonii
0.02	-	galangin	pH 6, 25°C	Penicillium olsonii
0.085	-	fisetin	pH 6, 25°C	Penicillium olsonii

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Cu	contains 0.9 copper atoms per protein	Penicillium olsonii

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
55000	-	1 * 55000, SDS-PAGE	Penicillium olsonii
56000	-	gel filtration	Penicillium olsonii

Organism

Organism	UniProt	Comment	Textmining
Penicillium olsonii	A7Y9J1	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	the purified enzyme is a mixture of at least four glycoforms	Penicillium olsonii

Purification (Commentary)

Purification (Comment)	Organism
-	Penicillium olsonii

Source Tissue

Source Tissue	Comment	Organism	Textmining
culture condition:rutin-grown cell	-	Penicillium olsonii	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
175	-	-	Penicillium olsonii

Storage Stability

Storage Stability	Organism
4°C, 6 months, the enzyme retains 81% of its initial activity	Penicillium olsonii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
fisetin + O2	16.8% of the activity with quercetin	Penicillium olsonii	?	-	?
galangin + O2	21% of the activity with quercetin	Penicillium olsonii	?	-	?
kaempferol + O2	activity is 2.18fold higher than with quercetin	Penicillium olsonii	?	-	?
additional information	flavonol, morin, 3,6-dihydroxyflavone and 3,7-dihydroxyflavone are transformed at a rate of less than 1% of that found for quercetin	Penicillium olsonii	?	-	?
quercetin + O2	-	Penicillium olsonii	2-(3,4-dihydroxybenzoyloxy)-4,6-dihydroxybenzoate + CO	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 55000, SDS-PAGE	Penicillium olsonii

Synonyms

Synonyms	Comment	Organism
quercetinase	-	Penicillium olsonii

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	quercetin oxidation	Penicillium olsonii

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
33.3	-	galangin	pH 6, 25°C	Penicillium olsonii
56.7	-	fisetin	pH 6, 25°C	Penicillium olsonii
166.7	-	quercetin	pH 6, 25°C	Penicillium olsonii
466.7	-	kaempferol	pH 6, 25°C	Penicillium olsonii

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	0.1 M MES buffer	Penicillium olsonii

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.004	-	1H-2-benzyl-3-hydroxy-4-oxoquinolin	pH 6, 25°C	Penicillium olsonii

pI Value

Organism	Comment	pI Value Maximum	pI Value
Penicillium olsonii	isoelectric focusing analysis of the purified enzyme reveals some heterogeneity since two major bands with apparent pI of 5.81 and 6.07 as well as two minor bands with apparent pI of 5.55 and 6.38 are observed. The purified enzyme is a mixture of at least four glycoforms	-	additional information

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Release 2023.1 (January 2023)