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Literature summary for 1.13.11.24 extracted from

  • Merkens, H.; Sielker, S.; Rose, K.; Fetzner, S.
    A new monocupin quercetinase of Streptomyces sp. FLA: identification and heterologous expression of the queD gene and activity of the recombinant enzyme towards different flavonols (2007), Arch. Microbiol., 187, 475-487.
    View publication on PubMed
Search for more literature for 1.13.11.24

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli or in in Streptomyces lividans TK23 Streptomyces sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0141
-
 quercetin
-
 Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23041
-
 x * 23041, calculated from sequence Streptomyces sp.
63000
-
 gel filtration Streptomyces sp.

Organism

Organism UniProt Comment Textmining
Streptomyces sp. A2VA43
-
-
Streptomyces sp. FLA / DSM 41951 A2VA43
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant QueD protein with a C-terminal hexahistidine-tag Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
fisetin + O2 i.e. 3,7,3',4'-tetrahydroxyflavone, 23% of the activity with quercetin Streptomyces sp. ?
-
 ?
fisetin + O2 i.e. 3,7,3',4'-tetrahydroxyflavone, 23% of the activity with quercetin Streptomyces sp. FLA / DSM 41951 ?
-
 ?
galangin + O2 i.e. 3,5,7-trihydroxyflavone, 28% of the activity with quercetin Streptomyces sp. ?
-
 ?
galangin + O2 i.e. 3,5,7-trihydroxyflavone, 28% of the activity with quercetin Streptomyces sp. FLA / DSM 41951 ?
-
 ?
kaempferol + O2 i.e. 3,5,7,4'-tetrahydroxyflavone. 70% of the activity with quercetin Streptomyces sp. ?
-
 ?
additional information no activity with luteolin Streptomyces sp. ?
-
 ?
additional information no activity with luteolin Streptomyces sp. FLA / DSM 41951 ?
-
 ?
morin + O2 i.e. 3,5,7,2',4'-pentahydroxyflavone, 1.7% of the activity with quercetin Streptomyces sp. ?
-
 ?
myricetin + O2 i.e. 3,5,7,3',4',5'-hexahydroxyflavone. 49% of the activity with quercetin Streptomyces sp. ?
-
 ?
myricetin + O2 i.e. 3,5,7,3',4',5'-hexahydroxyflavone. 49% of the activity with quercetin Streptomyces sp. FLA / DSM 41951 ?
-
 ?
quercetin + O2
-
 Streptomyces sp. 2-protocatechoylphloroglucinolcarboxylate + CO
-
 ?
quercetin + O2
-
 Streptomyces sp. FLA / DSM 41951 2-protocatechoylphloroglucinolcarboxylate + CO
-
 ?

Subunits

Subunits Comment Organism
? x * 23041, calculated from sequence Streptomyces sp.

Synonyms

Synonyms Comment Organism
QueD
-
 Streptomyces sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.45
-
 quercetin
-
 Streptomyces sp.