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Literature summary for 1.13.11.20 extracted from

  • Pierce, B.S.; Subedi, B.P.; Sardar, S.; Crowell, J.K.
    The ''Gln-Type'' thiol dioxygenase from Azotobacter vinelandii is a 3-mercaptopropionic acid dioxygenase (2015), Biochemistry, 54, 7477-7490 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.11.20

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
11.4
-
 L-cysteine pH 8.0, 25°C Azotobacter vinelandii
26
-
 cysteamine pH 8.0, 25°C Azotobacter vinelandii

Metals/Ions

Metals/Ions Comment Organism Structure
Iron iron content is 0.8 mol of iron per mol of MDO. Addition of NO to 3-mercaptopropanoate-bound enzyme quantitatively yields an iron-nitrosyl species with EPR features consistent with a mononuclear (FeNO)7 site Azotobacter vinelandii

Organism

Organism UniProt Comment Textmining
Azotobacter vinelandii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Azotobacter vinelandii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-mercaptopropanoate + O2 reaction of EC 1.13.11.91 Azotobacter vinelandii 3-sulfinopropanoate + H+
-
 ?
cysteamine + O2
-
 Azotobacter vinelandii ?
-
 ?
L-cysteine + O2
-
 Azotobacter vinelandii 3-sulfinoalanine
-
 ?
additional information enzyme exhibits a specificity for 3-mercaptopropanoate nearly 2 orders of magnitude greater than those for cysteine Azotobacter vinelandii ?
-
-

Subunits

Subunits Comment Organism
? x * 22696, calculated from sequence, x * 23000, SDS-PAGE Azotobacter vinelandii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.29
-
 cysteamine pH 8.0, 25°C Azotobacter vinelandii
1.2
-
 L-cysteine pH 8.0, 25°C Azotobacter vinelandii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
11
-
 cysteamine pH 8.0, 25°C Azotobacter vinelandii
110
-
 L-cysteine pH 8.0, 25°C Azotobacter vinelandii