Literature summary for 1.13.11.20 extracted from

Souness, R.J.; Kleffmann, T.; Tchesnokov, E.P.; Wilbanks, S.M.; Jameson, G.B.; Jameson, G.N.
Mechanistic implications of persulfenate and persulfide binding in the active site of cysteine dioxygenase (2013), Biochemistry, 52, 7606-7617.

Search for more literature for 1.13.11.20

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant expression of the thioredoxin-His6-tagged enzyme	Rattus norvegicus

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant enzyme complexed with cysteine persulfide or 3-mercaptopropionic acid persulfide, hanging drop vapor diffusion method, mixing of 0.0015 ml of 8 mg/ml protein with 0.0015 ml of reservoir solution containing 24-34% w/v PEG 4000, 100-250 mM ammonium acetate, 100 mM sodium citrate, pH 5.6, 0-4 mM dithionite, and 40 mM ligand, final pH is 6.1-6.2, 24°C, one week, X-ray diffraction structure determination and analysis at 1.63-2.05 A resolution	Rattus norvegicus

Crystallization (Comment)

Organism

purified recombinant enzyme complexed with cysteine persulfide or 3-mercaptopropionic acid persulfide, hanging drop vapor diffusion method, mixing of 0.0015 ml of 8 mg/ml protein with 0.0015 ml of reservoir solution containing 24-34% w/v PEG 4000, 100-250 mM ammonium acetate, 100 mM sodium citrate, pH 5.6, 0-4 mM dithionite, and 40 mM ligand, final pH is 6.1-6.2, 24°C, one week, X-ray diffraction structure determination and analysis at 1.63-2.05 A resolution

Rattus norvegicus

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	P21816	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant thioredoxin-His6-tagged enzyme by affinity chromatography	Rattus norvegicus

Synonyms

Synonyms	Comment	Organism
CDO	-	Rattus norvegicus

General Information

General Information	Comment	Organism
additional information	persulfenate and persulfide binding in the active site of cysteine dioxygenase, overview	Rattus norvegicus

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Release 2023.1 (January 2023)