Cloned (Comment) | Organism |
---|---|
heterologously expressed in human HepG2/C3A cells | Rattus norvegicus |
Crystallization (Comment) | Organism |
---|---|
X-ray crystal structure | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
C164A | mutations of nonessential residues has little effect | Rattus norvegicus |
C93S | mutant can not be converted to the mature form due to the loss of the cysteine residue involved in thioether crosslink formation | Rattus norvegicus |
H86A | inactive mutant, leading to loss of the His that serves as a metal ligand in the active site does not form any crosslink | Rattus norvegicus |
R60A | mutant forms with low activity, which has a markedly decreased affinity for cysteine, probably due to the loss of the hydrogen bonding partner for the carboxylate of the substrate, forms the crosslink more slowly | Rattus norvegicus |
S153A | mutations of nonessential residues has little effect | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | inhibitors of the 26S proteasome (e.g., proteasome inhibitor 1 and lactacystin) block CDO degradation in cysteine-deficient cells but had little or no effect on CDO concentration in hepatocytes cultured with excess cysteine | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Rattus norvegicus | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23000 | - |
SDS-PAGE | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rattus norvegicus | cysteine catabolism in mammals is dependent upon cysteine dioxygenase. System for regulation of cellular cysteine levels. Evidence of abnormal or deficient CDO activity has been reported in individuals with a variety of autoimmune and neurodegenerative diseases, including rheumatoid arthritis, Parkinsons disease, Alzheimers disease, and motor neuron diseases | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adipocyte | - |
Rattus norvegicus | - |
adipose tissue | - |
Rattus norvegicus | - |
hepatocyte | - |
Rattus norvegicus | - |
intestinal epithelium | CDO is found specifically in the mucus-secreting goblet cells | Rattus norvegicus | - |
liver | - |
Rattus norvegicus | - |
mammary gland | intense staining for CDO in ductal cells of pregnant rats but not in other mammary epithelial cells or in ductal cells of non-pregnant rats | Rattus norvegicus | - |
pancreas | exocrine cell, but not in islet endocrine cells | Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-cysteine + O2 | - |
Rattus norvegicus | 3-sulfino-L-alanine | - |
? | |
additional information | cysteine catabolism in mammals is dependent upon cysteine dioxygenase. System for regulation of cellular cysteine levels. Evidence of abnormal or deficient CDO activity has been reported in individuals with a variety of autoimmune and neurodegenerative diseases, including rheumatoid arthritis, Parkinsons disease, Alzheimers disease, and motor neuron diseases | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 23000, SDS-PAGE | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
CDO | - |
Rattus norvegicus |
cysteine dioxygenase | - |
Rattus norvegicus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Rattus norvegicus | isoelectric focusing | - |
5.8 |