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Literature summary for 1.13.11.20 extracted from

  • Stipanuk, M.H.; Ueki, I.; Dominy, J.E.; Simmons, C.R.; Hirschberger, L.L.
    Cysteine dioxygenase: a robust system for regulation of cellular cysteine levels (2009), Amino Acids, 37, 55-63.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
heterologously expressed in human HepG2/C3A cells Rattus norvegicus

Crystallization (Commentary)

Crystallization (Comment) Organism
X-ray crystal structure Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
C164A mutations of nonessential residues has little effect Rattus norvegicus
C93S mutant can not be converted to the mature form due to the loss of the cysteine residue involved in thioether crosslink formation Rattus norvegicus
H86A inactive mutant, leading to loss of the His that serves as a metal ligand in the active site does not form any crosslink Rattus norvegicus
R60A mutant forms with low activity, which has a markedly decreased affinity for cysteine, probably due to the loss of the hydrogen bonding partner for the carboxylate of the substrate, forms the crosslink more slowly Rattus norvegicus
S153A mutations of nonessential residues has little effect Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
additional information inhibitors of the 26S proteasome (e.g., proteasome inhibitor 1 and lactacystin) block CDO degradation in cysteine-deficient cells but had little or no effect on CDO concentration in hepatocytes cultured with excess cysteine Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Rattus norvegicus 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Rattus norvegicus cysteine catabolism in mammals is dependent upon cysteine dioxygenase. System for regulation of cellular cysteine levels. Evidence of abnormal or deficient CDO activity has been reported in individuals with a variety of autoimmune and neurodegenerative diseases, including rheumatoid arthritis, Parkinson’s disease, Alzheimer’s disease, and motor neuron diseases ?
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
adipocyte
-
Rattus norvegicus
-
adipose tissue
-
Rattus norvegicus
-
hepatocyte
-
Rattus norvegicus
-
intestinal epithelium CDO is found specifically in the mucus-secreting goblet cells Rattus norvegicus
-
liver
-
Rattus norvegicus
-
mammary gland intense staining for CDO in ductal cells of pregnant rats but not in other mammary epithelial cells or in ductal cells of non-pregnant rats Rattus norvegicus
-
pancreas exocrine cell, but not in islet endocrine cells Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-cysteine + O2
-
Rattus norvegicus 3-sulfino-L-alanine
-
?
additional information cysteine catabolism in mammals is dependent upon cysteine dioxygenase. System for regulation of cellular cysteine levels. Evidence of abnormal or deficient CDO activity has been reported in individuals with a variety of autoimmune and neurodegenerative diseases, including rheumatoid arthritis, Parkinson’s disease, Alzheimer’s disease, and motor neuron diseases Rattus norvegicus ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 23000, SDS-PAGE Rattus norvegicus

Synonyms

Synonyms Comment Organism
CDO
-
Rattus norvegicus
cysteine dioxygenase
-
Rattus norvegicus

pI Value

Organism Comment pI Value Maximum pI Value
Rattus norvegicus isoelectric focusing
-
5.8