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Literature summary for 1.13.11.20 extracted from

  • Dominy, J.E.; Hwang, J.; Stipanuk, M.H.
    Overexpression of cysteine dioxygenase reduces intracellular cysteine and glutathione pools in HepG2/C3A cells (2007), Am. J. Physiol. Endocrinol. Metab., 293, E62-E69.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
cysteine protein expression of recombinant wild-type enzyme in HepG2/C3A cells increases by 160% when extracellular cysteine levels are increased from 0 to 1 mM cysteine Rattus norvegicus

Cloned(Commentary)

Cloned (Comment) Organism
expression in HepG2/C3A cell Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
H86A expression of either the wild-type or a catalytically inactivated mutant H86A in HepG2/C3A cells which do not express endogenous cysteine dioxygenase protein and culture in different concentrations of extracellular cysteine. Wild-type enzyme, but not mutant H86A, is capable of reducing intracellular cysteine levels in cells incubated in physiologically relevant concentrations of cysteine. Wild-type enzyme also decreases the glutathione pool and potentiates the toxicity of CdCl2 Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
CdCl2 cells transfected with wild-type enzyme show an enhanced sensitivity to CdCl2 that is limited to cells cultured in medium with cysteine levels of 0.1 and 0.3mM Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22500
-
x * 22500, calculated Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Subunits

Subunits Comment Organism
? x * 22500, calculated Rattus norvegicus