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Literature summary for 1.13.11.2 extracted from

  • Wojcieszynska, D.; Hupert-Kocurek, K.; Gren, I.; Guzik, U.
    High activity catechol 2,3-dioxygenase from the cresols - degrading Stenotrophomonas maltophilia strain KB2 (2011), Int. Biodeter. Biodegrad., 65, 853-858.
No PubMed abstract available
Search for more literature for 1.13.11.2

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis Stenotrophomonas maltophilia

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ Fe2þ ion binding is facilitated via four ligands: two histidine residues, one glutamate residue and one molecule of water, overview Stenotrophomonas maltophilia

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-methylphenol + O2 Stenotrophomonas maltophilia
-
 ?
-
 ?
2-methylphenol + O2 Stenotrophomonas maltophilia KB2
-
 ?
-
 ?
3-methylphenol + O2 Stenotrophomonas maltophilia
-
 ?
-
 ?
3-methylphenol + O2 Stenotrophomonas maltophilia KB2
-
 ?
-
 ?
4-Methylphenol + O2 Stenotrophomonas maltophilia
-
 ?
-
 ?
4-Methylphenol + O2 Stenotrophomonas maltophilia KB2
-
 ?
-
 ?
catechol + O2 Stenotrophomonas maltophilia
-
 2-hydroxymuconate-6-semialdehyde
-
 ?
catechol + O2 Stenotrophomonas maltophilia KB2
-
 2-hydroxymuconate-6-semialdehyde
-
 ?

Organism

Organism UniProt Comment Textmining
Stenotrophomonas maltophilia A7LB47
-
-
Stenotrophomonas maltophilia KB2 A7LB47
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-methylphenol + O2
-
 Stenotrophomonas maltophilia ?
-
 ?
2-methylphenol + O2
-
 Stenotrophomonas maltophilia KB2 ?
-
 ?
3-methylphenol + O2
-
 Stenotrophomonas maltophilia ?
-
 ?
3-methylphenol + O2
-
 Stenotrophomonas maltophilia KB2 ?
-
 ?
4-Methylphenol + O2
-
 Stenotrophomonas maltophilia ?
-
 ?
4-Methylphenol + O2
-
 Stenotrophomonas maltophilia KB2 ?
-
 ?
catechol + O2
-
 Stenotrophomonas maltophilia 2-hydroxymuconate-6-semialdehyde
-
 ?
catechol + O2
-
 Stenotrophomonas maltophilia KB2 2-hydroxymuconate-6-semialdehyde
-
 ?

Subunits

Subunits Comment Organism
More the secondary structure shows presence of externally located alpha-helices and internally located beta-sheets, enzyme molecular modeling, overview Stenotrophomonas maltophilia

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Stenotrophomonas maltophilia

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
 loss of 17.4% activity Stenotrophomonas maltophilia
35 40 rapid decline in activity Stenotrophomonas maltophilia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
-
 Stenotrophomonas maltophilia

Expression

Organism Comment Expression
Stenotrophomonas maltophilia the enzyme in strain KB2 is induced by growth on methylphenols up

General Information

General Information Comment Organism
evolution the enzyme belongs to the single-ring substrate subfamily of the extradiol dioxygenase Stenotrophomonas maltophilia