Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.13.11.2 extracted from

  • Jiang, Y.; Yang, X.; Liu, B.; Zhao, H.; Cheng, Q.; Cai, B.
    Catechol 2,3-dioxygenase from Pseudomonas sp. strain ND6: Gene sequence and enzyme characterization (2004), Biosci. Biotechnol. Biochem., 68, 1798-1800.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DH 5alpha Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
catechol 57°C Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
SDS-PAGE Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
catechol + O2 Pseudomonas sp. cleavage of aromatic compounds, involved in alpha-ketoadiapate pathway 2-hydroxymuconate semialdehyde
-
?
catechol + O2 Pseudomonas sp. ND6 cleavage of aromatic compounds, involved in alpha-ketoadiapate pathway 2-hydroxymuconate semialdehyde
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. ND6
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
catechol + O2
-
Pseudomonas sp. 2-hydroxymuconate semialdehyde
-
?
catechol + O2 cleavage of aromatic compounds, involved in alpha-ketoadiapate pathway Pseudomonas sp. 2-hydroxymuconate semialdehyde
-
?
catechol + O2
-
Pseudomonas sp. ND6 2-hydroxymuconate semialdehyde
-
?
catechol + O2 cleavage of aromatic compounds, involved in alpha-ketoadiapate pathway Pseudomonas sp. ND6 2-hydroxymuconate semialdehyde
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
57
-
63% loss of activity within 10 min Pseudomonas sp.