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Literature summary for 1.13.11.2 extracted from

  • Okuta, A.; Ohnishi, K.; Yagame, S.; Harayama, S.
    Intersubunit interaction and catalytic activity of catechol 2,3-dioxygenases (2003), Biochem. J., 371, 557-564.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.11.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Pseudomonas sp.
expressed in Escherichia coli Sphingomonas sp.

Protein Variants

Protein Variants Comment Organism
additional information construction of different hybrid enzymes containing different parts of type I.2.A and I.2.B enzyme, only one of the constructed enzymes is found to be active Pseudomonas sp.
additional information construction of different hybrid enzymes containing different parts of type I.2.A and I.2.B enzyme, only one of the constructed enzymes is found to be active Sphingomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0029
-
 catechol native enzyme, pH 7.5, 25°C Pseudomonas sp.
0.0038
-
 catechol native enzyme, pH 7.5, 25°C Sphingomonas sp.
0.02
-
 catechol hybrid enzyme, pH 7.5, 25°C Pseudomonas sp.
0.02
-
 catechol hybrid enzyme, pH 7.5, 25°C Sphingomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Iron bound to protein Pseudomonas sp.
Iron bound to protein Sphingomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
 4 * 37000, SDS-PAGE Pseudomonas sp.
37000
-
 4 * 37000, SDS-PAGE Sphingomonas sp.
150000
-
 gel filtration Pseudomonas sp.
150000
-
 gel filtration Sphingomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
catechol + O2 Pseudomonas sp. key enzyme for the degradation of aromatic compounds 2-hydroxymuconate semialdehyde
-
 ?
catechol + O2 Sphingomonas sp. key enzyme for the degradation of aromatic compounds 2-hydroxymuconate semialdehyde
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
 enzyme subgroup I.2.A, hybrid enzymes with type I.2.B enzyme from Sphingomonas sp.
-
Sphingomonas sp.
-
 enzyme subgroup I.2.B, hybrid enzymes with type I.2.A enzyme from Pseudomonas sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
catechol + O2
-
 Pseudomonas sp. 2-hydroxymuconate semialdehyde
-
 ?
catechol + O2
-
 Sphingomonas sp. 2-hydroxymuconate semialdehyde
-
 ?
catechol + O2 key enzyme for the degradation of aromatic compounds Pseudomonas sp. 2-hydroxymuconate semialdehyde
-
 ?
catechol + O2 key enzyme for the degradation of aromatic compounds Sphingomonas sp. 2-hydroxymuconate semialdehyde
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 37000, SDS-PAGE Pseudomonas sp.
tetramer 4 * 37000, SDS-PAGE Sphingomonas sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
148
-
 catechol hybrid enzyme, pH 7.5, 25°C Pseudomonas sp.
148
-
 catechol hybrid enzyme, pH 7.5, 25°C Sphingomonas sp.
238
-
 catechol native enzyme, pH 7.5, 25°C Sphingomonas sp.
493
-
 catechol native enzyme, pH 7.5, 25°C Pseudomonas sp.