Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas sp. |
expression in Escherichia coli | Pseudomonas putida |
expression in Escherichia coli | Ralstonia pickettii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km determination for enzymes from hypoxic and nonhypoxic pseudomonads, Km for oxygen approximately five-fold lower under hypoxic conditions for hypoxic strains | Pseudomonas fluorescens | |
additional information | - |
additional information | Km determination for enzymes from hypoxic and nonhypoxic pseudomonads, Km for oxygen approximately five-fold lower under hypoxic conditions for hypoxic strains | Pseudomonas sp. | |
additional information | - |
additional information | Km determination for enzymes from hypoxic and nonhypoxic pseudomonads, Km for oxygen approximately five-fold lower under hypoxic conditions for hypoxic strains | Pseudomonas putida | |
additional information | - |
additional information | Km determination for enzymes from hypoxic and nonhypoxic pseudomonads, Km for oxygen approximately five-fold lower under hypoxic conditions for hypoxic strains | Burkholderia cepacia | |
additional information | - |
additional information | Km determination for enzymes from hypoxic and nonhypoxic pseudomonads, Km for oxygen approximately five-fold lower under hypoxic conditions for hypoxic strains | Ralstonia pickettii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
catechol + O2 | Burkholderia cepacia | - |
2-hydroxymuconate semialdehyde | - |
? | |
catechol + O2 | Pseudomonas fluorescens | enzyme is a important component in the degradation pathways of toluene and xylene and catalyses the dioxygenolytic cleavage of the aromatic ring | 2-hydroxymuconate semialdehyde | - |
? | |
catechol + O2 | Pseudomonas sp. | enzyme is a important component in the degradation pathways of toluene and xylene and catalyses the dioxygenolytic cleavage of the aromatic ring | 2-hydroxymuconate semialdehyde | - |
? | |
catechol + O2 | Pseudomonas putida | enzyme is a important component in the degradation pathways of toluene and xylene and catalyses the dioxygenolytic cleavage of the aromatic ring | 2-hydroxymuconate semialdehyde | - |
? | |
catechol + O2 | Ralstonia pickettii | enzyme is a important component in the degradation pathways of toluene and xylene and catalyses the dioxygenolytic cleavage of the aromatic ring | 2-hydroxymuconate semialdehyde | - |
? | |
catechol + O2 | Pseudomonas fluorescens CFS 215 | enzyme is a important component in the degradation pathways of toluene and xylene and catalyses the dioxygenolytic cleavage of the aromatic ring | 2-hydroxymuconate semialdehyde | - |
? | |
catechol + O2 | Ralstonia pickettii PK01 | enzyme is a important component in the degradation pathways of toluene and xylene and catalyses the dioxygenolytic cleavage of the aromatic ring | 2-hydroxymuconate semialdehyde | - |
? | |
catechol + O2 | Burkholderia cepacia G4 | - |
2-hydroxymuconate semialdehyde | - |
? | |
catechol + O2 | Pseudomonas sp. W31 | enzyme is a important component in the degradation pathways of toluene and xylene and catalyses the dioxygenolytic cleavage of the aromatic ring | 2-hydroxymuconate semialdehyde | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia cepacia | - |
- |
- |
Burkholderia cepacia G4 | - |
- |
- |
Pseudomonas fluorescens | - |
- |
- |
Pseudomonas fluorescens CFS 215 | - |
- |
- |
Pseudomonas putida | - |
F1 and PaW1 | - |
Pseudomonas sp. | - |
W31 | - |
Pseudomonas sp. W31 | - |
W31 | - |
Ralstonia pickettii | - |
- |
- |
Ralstonia pickettii PK01 | - |
- |
- |
Oxidation Stability | Organism |
---|---|
significant nitrate-dependent enhanced degradation of toluene under hypoxic i.e. oxygen-limited condition | Pseudomonas fluorescens |
significant nitrate-dependent enhanced degradation of toluene under hypoxic i.e. oxygen-limited condition | Pseudomonas sp. |
significant nitrate-dependent enhanced degradation of toluene under hypoxic i.e. oxygen-limited condition | Ralstonia pickettii |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas fluorescens |
- |
Pseudomonas sp. |
- |
Ralstonia pickettii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-methylcatechol + O2 | - |
Pseudomonas fluorescens | 2-hydroxy-6-oxohepta-2,4-dienoate | - |
? | |
3-methylcatechol + O2 | - |
Pseudomonas sp. | 2-hydroxy-6-oxohepta-2,4-dienoate | - |
? | |
3-methylcatechol + O2 | - |
Pseudomonas putida | 2-hydroxy-6-oxohepta-2,4-dienoate | - |
? | |
3-methylcatechol + O2 | - |
Burkholderia cepacia | 2-hydroxy-6-oxohepta-2,4-dienoate | - |
? | |
3-methylcatechol + O2 | - |
Ralstonia pickettii | 2-hydroxy-6-oxohepta-2,4-dienoate | - |
? | |
3-methylcatechol + O2 | - |
Pseudomonas fluorescens CFS 215 | 2-hydroxy-6-oxohepta-2,4-dienoate | - |
? | |
3-methylcatechol + O2 | - |
Ralstonia pickettii PK01 | 2-hydroxy-6-oxohepta-2,4-dienoate | - |
? | |
3-methylcatechol + O2 | - |
Burkholderia cepacia G4 | 2-hydroxy-6-oxohepta-2,4-dienoate | - |
? | |
3-methylcatechol + O2 | - |
Pseudomonas sp. W31 | 2-hydroxy-6-oxohepta-2,4-dienoate | - |
? | |
4-methylcatechol + O2 | hypoxic strains with significantly higher affinities | Pseudomonas fluorescens | 2-hydroxy-3-methyl-6-oxohexa-2,4-dienoate | - |
? | |
4-methylcatechol + O2 | hypoxic strains with significantly higher affinities | Pseudomonas sp. | 2-hydroxy-3-methyl-6-oxohexa-2,4-dienoate | - |
? | |
4-methylcatechol + O2 | hypoxic strains with significantly higher affinities | Pseudomonas putida | 2-hydroxy-3-methyl-6-oxohexa-2,4-dienoate | - |
? | |
4-methylcatechol + O2 | hypoxic strains with significantly higher affinities | Burkholderia cepacia | 2-hydroxy-3-methyl-6-oxohexa-2,4-dienoate | - |
? | |
4-methylcatechol + O2 | hypoxic strains with significantly higher affinities | Ralstonia pickettii | 2-hydroxy-3-methyl-6-oxohexa-2,4-dienoate | - |
? | |
4-methylcatechol + O2 | hypoxic strains with significantly higher affinities | Pseudomonas fluorescens CFS 215 | 2-hydroxy-3-methyl-6-oxohexa-2,4-dienoate | - |
? | |
4-methylcatechol + O2 | hypoxic strains with significantly higher affinities | Ralstonia pickettii PK01 | 2-hydroxy-3-methyl-6-oxohexa-2,4-dienoate | - |
? | |
4-methylcatechol + O2 | hypoxic strains with significantly higher affinities | Burkholderia cepacia G4 | 2-hydroxy-3-methyl-6-oxohexa-2,4-dienoate | - |
? | |
4-methylcatechol + O2 | hypoxic strains with significantly higher affinities | Pseudomonas sp. W31 | 2-hydroxy-3-methyl-6-oxohexa-2,4-dienoate | - |
? | |
catechol + O2 | - |
Burkholderia cepacia | 2-hydroxymuconate semialdehyde | - |
? | |
catechol + O2 | hypoxic strains have enzymes with significantly higher affinities for catechol than for nonhypoxic strains | Pseudomonas fluorescens | 2-hydroxymuconate semialdehyde | - |
? | |
catechol + O2 | hypoxic strains have enzymes with significantly higher affinities for catechol than for nonhypoxic strains | Pseudomonas sp. | 2-hydroxymuconate semialdehyde | - |
? | |
catechol + O2 | hypoxic strains have enzymes with significantly higher affinities for catechol than for nonhypoxic strains | Pseudomonas putida | 2-hydroxymuconate semialdehyde | - |
? | |
catechol + O2 | hypoxic strains have enzymes with significantly higher affinities for catechol than for nonhypoxic strains | Burkholderia cepacia | 2-hydroxymuconate semialdehyde | - |
? | |
catechol + O2 | hypoxic strains have enzymes with significantly higher affinities for catechol than for nonhypoxic strains | Ralstonia pickettii | 2-hydroxymuconate semialdehyde | - |
? | |
catechol + O2 | enzyme is a important component in the degradation pathways of toluene and xylene and catalyses the dioxygenolytic cleavage of the aromatic ring | Pseudomonas fluorescens | 2-hydroxymuconate semialdehyde | - |
? | |
catechol + O2 | enzyme is a important component in the degradation pathways of toluene and xylene and catalyses the dioxygenolytic cleavage of the aromatic ring | Pseudomonas sp. | 2-hydroxymuconate semialdehyde | - |
? | |
catechol + O2 | enzyme is a important component in the degradation pathways of toluene and xylene and catalyses the dioxygenolytic cleavage of the aromatic ring | Pseudomonas putida | 2-hydroxymuconate semialdehyde | - |
? | |
catechol + O2 | enzyme is a important component in the degradation pathways of toluene and xylene and catalyses the dioxygenolytic cleavage of the aromatic ring | Ralstonia pickettii | 2-hydroxymuconate semialdehyde | - |
? | |
catechol + O2 | hypoxic strains have enzymes with significantly higher affinities for catechol than for nonhypoxic strains | Pseudomonas fluorescens CFS 215 | 2-hydroxymuconate semialdehyde | - |
? | |
catechol + O2 | enzyme is a important component in the degradation pathways of toluene and xylene and catalyses the dioxygenolytic cleavage of the aromatic ring | Pseudomonas fluorescens CFS 215 | 2-hydroxymuconate semialdehyde | - |
? | |
catechol + O2 | hypoxic strains have enzymes with significantly higher affinities for catechol than for nonhypoxic strains | Ralstonia pickettii PK01 | 2-hydroxymuconate semialdehyde | - |
? | |
catechol + O2 | enzyme is a important component in the degradation pathways of toluene and xylene and catalyses the dioxygenolytic cleavage of the aromatic ring | Ralstonia pickettii PK01 | 2-hydroxymuconate semialdehyde | - |
? | |
catechol + O2 | - |
Burkholderia cepacia G4 | 2-hydroxymuconate semialdehyde | - |
? | |
catechol + O2 | hypoxic strains have enzymes with significantly higher affinities for catechol than for nonhypoxic strains | Burkholderia cepacia G4 | 2-hydroxymuconate semialdehyde | - |
? | |
catechol + O2 | hypoxic strains have enzymes with significantly higher affinities for catechol than for nonhypoxic strains | Pseudomonas sp. W31 | 2-hydroxymuconate semialdehyde | - |
? | |
catechol + O2 | enzyme is a important component in the degradation pathways of toluene and xylene and catalyses the dioxygenolytic cleavage of the aromatic ring | Pseudomonas sp. W31 | 2-hydroxymuconate semialdehyde | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the hypoxic strains have enzymes with significantly higher substrate turnover rates for the nonhypoxic strains | Pseudomonas fluorescens | |
additional information | - |
additional information | the hypoxic strains have enzymes with significantly higher substrate turnover rates for the nonhypoxic strains | Pseudomonas sp. | |
additional information | - |
additional information | the hypoxic strains have enzymes with significantly higher substrate turnover rates for the nonhypoxic strains | Pseudomonas putida | |
additional information | - |
additional information | the hypoxic strains have enzymes with significantly higher substrate turnover rates for the nonhypoxic strains | Burkholderia cepacia | |
additional information | - |
additional information | the hypoxic strains have enzymes with significantly higher substrate turnover rates for the nonhypoxic strains | Ralstonia pickettii |