Literature summary for 1.13.11.2 extracted from

Cerdan, P.; Rekik, M.; Harayama, S.
Substrate specificity differences between two catechol 2,3-dioxygenases encoded by the TOL and NAH plasmids from Pseudomonas putida (1995), Eur. J. Biochem., 229, 113-118.

Search for more literature for 1.13.11.2

Cloned(Commentary)

Cloned (Comment)	Organism
-	Escherichia coli
-	Pseudomonas putida

Protein Variants

Protein Variants	Comment	Organism
H250G	reduced twofold relative kcat value for 3-methylcatechol	Escherichia coli
H250G	reduced twofold relative kcat value for 3-methylcatechol	Pseudomonas putida

Inhibitors

Inhibitors	Comment	Organism	Structure
3-methylcatechol	suicide inhibition	Escherichia coli
3-methylcatechol	suicide inhibition	Pseudomonas putida
4-methylcatechol	suicide inhibition	Escherichia coli
4-methylcatechol	suicide inhibition	Pseudomonas putida
catechol	suicide inhibition	Escherichia coli
catechol	suicide inhibition	Pseudomonas putida

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0014	-	catechol	-	Pseudomonas putida
0.0017	-	4-methylcatechol	-	Pseudomonas putida
0.0018	-	3-methylcatechol	-	Pseudomonas putida
0.0024	-	3-methylcatechol	-	Escherichia coli
0.004	-	catechol	-	Escherichia coli
0.006	-	4-methylcatechol	-	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-
Escherichia coli JM83	-	-	-
Pseudomonas putida	-	KT 2440	-
Pseudomonas putida KT 2440	-	KT 2440	-

Purification (Commentary)

Purification (Comment)	Organism
-	Escherichia coli
-	Pseudomonas putida

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3-methylcatechol + O2	-	Escherichia coli	2-hydroxy-6-oxohepta-2,4-dienoate	-	?
3-methylcatechol + O2	-	Pseudomonas putida	2-hydroxy-6-oxohepta-2,4-dienoate	-	?
3-methylcatechol + O2	-	Pseudomonas putida KT 2440	2-hydroxy-6-oxohepta-2,4-dienoate	-	?
3-methylcatechol + O2	-	Escherichia coli JM83	2-hydroxy-6-oxohepta-2,4-dienoate	-	?
4-methylcatechol + O2	-	Escherichia coli	2-hydroxy-3-methyl-6-oxohexa-2,4-dienoate	-	?
4-methylcatechol + O2	-	Pseudomonas putida	2-hydroxy-3-methyl-6-oxohexa-2,4-dienoate	-	?
4-methylcatechol + O2	-	Pseudomonas putida KT 2440	2-hydroxy-3-methyl-6-oxohexa-2,4-dienoate	-	?
4-methylcatechol + O2	-	Escherichia coli JM83	2-hydroxy-3-methyl-6-oxohexa-2,4-dienoate	-	?
catechol + O2	-	Escherichia coli	2-hydroxymuconate semialdehyde	-	?
catechol + O2	-	Pseudomonas putida	2-hydroxymuconate semialdehyde	-	?
catechol + O2	-	Pseudomonas putida KT 2440	2-hydroxymuconate semialdehyde	-	?
catechol + O2	-	Escherichia coli JM83	2-hydroxymuconate semialdehyde	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
additional information	-	additional information	kcat values of enzyme and hybrid proteins	Escherichia coli
additional information	-	additional information	kcat values of enzyme and hybrid proteins	Pseudomonas putida
190	-	3-methylcatechol	-	Escherichia coli
490	-	3-methylcatechol	-	Pseudomonas putida
690	-	catechol	-	Escherichia coli
930	-	catechol	-	Pseudomonas putida
930	-	4-methylcatechol	-	Escherichia coli
930	-	4-methylcatechol	-	Pseudomonas putida

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Release 2023.1 (January 2023)