Any feedback?
Literature summary for 1.13.11.16 extracted from
- Overproduction, purification and properties of 2,3-dihydroxyphenylpropionate 1,2-dioxygenase from Escherichia coli (1993), Biochim. Biophys. Acta, 1202, 258-264.
General Stability
| General Stability | Organism |
|---|---|
| activity stable in crude extract, inactive apoenzyme stable, can be re-activated by treatment with Fe2+ and ascorbate, irreversible apoenzyme activity loss if stored in completely Fe2+-free buffer, re-activated holoenzyme unstable | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Escherichia coli |  |
| H2O2 | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.026 | - |
3-(2,3-dihydroxyphenyl)propionate | - |
Escherichia coli | |
| 0.09 | - |
3-methylcatechol | - |
Escherichia coli | |
| 0.72 | - |
catechol | - |
Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | weakly bound, 1 mol per mol tetramer, by addition of Fe2+ re-activated apoenzyme | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
4 * 36000, SDS-PAGE | Escherichia coli |
| 134000 | - |
gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-(2,3-dihydroxyphenyl)propionate + O2 | Escherichia coli | - |
2-hydroxy-6-oxonona-2,4-diene-1,9-dioate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P0ABR9 | gene mhpB | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 47.9 | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,3-dihydroxyphenylpropionic acid + O2 | - |
Escherichia coli | 2-hydroxy-6-oxonona-dienoate | - |
? | |
| 3-(2,3-dihydroxyphenyl)propionate + O2 | - |
Escherichia coli | 2-hydroxy-6-oxonona-2,4-diene-1,9-dioate | - |
? | |
| 3-methylcatechol + O2 | meta-ring cleavage | Escherichia coli | 2-hydroxy-6-oxohepta-2,4-dienoate | - |
? | |
| catechol + O2 | meta-ring-cleavage | Escherichia coli | 2-hydroxy-6-oxohexa-2,4-dienoate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 36000, SDS-PAGE | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2 | - |
catechol | - |
Escherichia coli | |
| 3.6 | - |
3-methylcatechol | - |
Escherichia coli | |
| 29 | - |
3-(2,3-dihydroxyphenyl)propionate | - |
Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9.5 | - |
Escherichia coli |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
