Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Brevibacterium fuscum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | triple mutant in which the three gaps in the sequence are removed does not show a significant change in metal content | Brevibacterium fuscum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
3,4-Dihydroxyphenylacetate | recombinant enzyme | Brevibacterium fuscum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | recombinant enzyme contains 2.8 mol of iron per mol of enzyme | Brevibacterium fuscum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dihydroxyphenylacetate + O2 | Brevibacterium fuscum | key ring cleavage step in metabolism of 3,4-dihydroxyphenylacetate | 2-hydroxy-5-carboxymethylmuconate semialdehyde | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brevibacterium fuscum | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.2 | - |
recombinant enzyme | Brevibacterium fuscum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dihydroxyphenylacetate + O2 | - |
Brevibacterium fuscum | 2-hydroxy-5-carboxymethylmuconate semialdehyde | - |
? | |
3,4-dihydroxyphenylacetate + O2 | key ring cleavage step in metabolism of 3,4-dihydroxyphenylacetate | Brevibacterium fuscum | 2-hydroxy-5-carboxymethylmuconate semialdehyde | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | turnover-numbers of recombinant wild-type enzyme and mutant enzyme | Brevibacterium fuscum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
recombinant wild-type enzyme and mutant enzyme | Brevibacterium fuscum |