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Literature summary for 1.13.11.15 extracted from
- Homoprotocatechuate 2,3-dioxygenase from Brevibacterium fuscum. A dioxygenase with catalase activity (1996), J. Biol. Chem., 271, 5524-5535.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| H2O2 | stable to H2O2, due to the fact that the enzyme is also an effective catalase | Brevibacterium fuscum |  |
| K3Fe(CN)6 | 10 mM, 35% inhibition | Brevibacterium fuscum | |
| additional information | stable to H2O2, due to the fact that the enzyme is also an effective catalase | Brevibacterium fuscum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.015 | - |
3,4-Dihydroxyphenylacetate | - |
Brevibacterium fuscum | |
| 0.06 | - |
O2 | - |
Brevibacterium fuscum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | Fe(II)-containing, contains 0.9 gatom of iron per subunit | Brevibacterium fuscum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42500 | - |
4 * 42500, or 6 * 42500, SDS-PAGE | Brevibacterium fuscum |
| 42500 | - |
6 * 42500, or 42500, SDS-PAGE | Brevibacterium fuscum |
| 220000 | - |
equilibrium sedimentation | Brevibacterium fuscum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Brevibacterium fuscum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Brevibacterium fuscum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Brevibacterium fuscum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3,4-dihydroxyphenylacetate + O2 | - |
Brevibacterium fuscum | 2-hydroxy-5-carboxymethylmuconate semialdehyde | - |
? | |
| additional information | enzyme also has catalase activity | Brevibacterium fuscum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 42500, or 42500, SDS-PAGE | Brevibacterium fuscum |
| tetramer | 4 * 42500, or 6 * 42500, SDS-PAGE | Brevibacterium fuscum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Brevibacterium fuscum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Brevibacterium fuscum |
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