Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | 0.1 mM, 13% inhibition | Geobacillus stearothermophilus | |
Ferrous ammonium sulfate | 0.1 mM, 32% inhibition | Geobacillus stearothermophilus | |
p-hydroxymercuribenzoate | 0.1 mM, 13% inhibition | Geobacillus stearothermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0034 | - |
3,4-Dihydroxyphenylacetate | 32°C | Geobacillus stearothermophilus | |
0.0037 | - |
3,4-Dihydroxyphenylpropionic acid | 32°C | Geobacillus stearothermophilus | |
0.004 | - |
3,4-Dihydroxyphenylacetate | 28°C | Geobacillus stearothermophilus | |
0.0047 | - |
3,4-Dihydroxyphenylacetate | 21°C | Geobacillus stearothermophilus | |
0.0058 | - |
3,4-Dihydroxyphenylacetate | 38°C | Geobacillus stearothermophilus | |
0.0074 | - |
3,4-Dihydroxyphenylacetate | 47°C | Geobacillus stearothermophilus | |
0.035 | - |
DL-3,4-dihydroxymandelic acid | 32°C | Geobacillus stearothermophilus | |
0.45 | - |
3,4-dihydroxybenzoic acid | - |
Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
106000 | - |
gel filtration | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Geobacillus stearothermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.25 | - |
- |
Geobacillus stearothermophilus |
Storage Stability | Organism |
---|---|
room temperature, little loss of activity after 3 months | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dihydroxybenzoic acid + O2 | 0.58% of the activity with 3,4-dihydroxyphenylacetate | Geobacillus stearothermophilus | 2-hydroxy-5-carboxymuconate semialdehyde | - |
? | |
3,4-dihydroxymandelate + O2 | 83% of the activity with 3,4-dihydroxyphenylacetate | Geobacillus stearothermophilus | ? | - |
? | |
3,4-dihydroxyphenylacetate + O2 | - |
Geobacillus stearothermophilus | 2-hydroxy-5-carboxymethylmuconate semialdehyde | - |
? | |
3,4-dihydroxyphenylpropionate + O2 | 58% of the activity with 3,4-dihydroxyphenylacetate | Geobacillus stearothermophilus | 2-hydroxy-5-carboxyethylmuconate semialdehyde | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 33000-35000 | Geobacillus stearothermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
57 | - |
at pH 8.2 | Geobacillus stearothermophilus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 64 | 40°C: about 40% of maximal activity, 64°C: about 75% of maximal activity | Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
63 | - |
2 h, 5% loss of activity | Geobacillus stearothermophilus |
88 | - |
10 min, 50% loss of activity | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.4 | 8.7 | - |
Geobacillus stearothermophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.1 | 9.3 | 78% of maximal activity at pH 7.1 and 9.3 | Geobacillus stearothermophilus |