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Literature summary for 1.13.11.12 extracted from

  • Cao, S.; Chen, H.; Zhang, C.; Tang, Y.; Liu, J.; Qi, H.
    Heterologous expression and biochemical characterization of two lipoxygenases in oriental melon, Cucumis melo var. makuwa Makino (2016), PLoS ONE, 11, e0153801 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.11.12

Cloned(Commentary)

Cloned (Comment) Organism
gene CmLOX10, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, recombinant expression of His-tagged full-length enzyme in Escherichia coli strain BL21(DE3) Cucumis melo var. makuwa
gene CmLOX13, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, recombinant expression of His-tagged truncated enzyme in Escherichia coli strain BL21(DE3) Cucumis melo var. makuwa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1258
-
 linoleic acid pH 5.0, 45°C, recombinant Hi-tagged enzyme Cucumis melo var. makuwa
0.1402
-
 linolenic acid pH 5.0, 45°C, recombinant Hi-tagged enzyme Cucumis melo var. makuwa
0.1566
-
 linolenic acid pH 5.0, 45°C, recombinant Hi-tagged enzyme Cucumis melo var. makuwa
0.2303
-
 linoleic acid pH 5.0, 45°C, recombinant Hi-tagged enzyme Cucumis melo var. makuwa

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Cucumis melo var. makuwa 9507
-
plasma membrane
-
 Cucumis melo var. makuwa 5886
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
linoleic acid + O2 Cucumis melo var. makuwa
-
 (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoate
-
 ?
linolenic acid + O2 Cucumis melo var. makuwa
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Cucumis melo var. makuwa A0A125S6K7 cultivar Yumeiren
-
Cucumis melo var. makuwa A0A125S6K8 cultivar Yumeiren
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged full-length enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Cucumis melo var. makuwa
recombinant His-tagged truncated enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Cucumis melo var. makuwa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
linoleic acid + O2
-
 Cucumis melo var. makuwa (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoate
-
 ?
linolenic acid + O2
-
 Cucumis melo var. makuwa ?
-
 ?

Subunits

Subunits Comment Organism
? x * 102301, sequence calculation Cucumis melo var. makuwa
? x * 102312, sequence calculation Cucumis melo var. makuwa
More enzyme domain structure, overview Cucumis melo var. makuwa

Synonyms

Synonyms Comment Organism
CmLOX10
-
 Cucumis melo var. makuwa
CmLOX13
-
 Cucumis melo var. makuwa
LOX
-
 Cucumis melo var. makuwa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Cucumis melo var. makuwa
45
-
-
 Cucumis melo var. makuwa

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 50 enzyme activity of CmLOX10 increases as temperature rises between 20°C and 45°C and then decreases at 50°C, profile overview Cucumis melo var. makuwa
20 50 enzyme activity of CmLOX13 is sharply decreased when the temperature is higher than 35°C, profile overview Cucumis melo var. makuwa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1841.7
-
 linolenic acid pH 5.0, 45°C, recombinant Hi-tagged enzyme Cucumis melo var. makuwa
2438.6
-
 linoleic acid pH 5.0, 45°C, recombinant Hi-tagged enzyme Cucumis melo var. makuwa
2442.96
-
 linolenic acid pH 5.0, 45°C, recombinant Hi-tagged enzyme Cucumis melo var. makuwa
5492.88
-
 linoleic acid pH 5.0, 45°C, recombinant Hi-tagged enzyme Cucumis melo var. makuwa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Cucumis melo var. makuwa
5.5
-
-
 Cucumis melo var. makuwa

pI Value

Organism Comment pI Value Maximum pI Value
Cucumis melo var. makuwa sequence calculation
-
 6.1
Cucumis melo var. makuwa sequence calculation
-
 6.34

General Information

General Information Comment Organism
evolution genes CmLOX10 and CmLOX13 include all of the typical LOX domains and share 58.11% identity at the amino acid level with each other. The phylogenetic analysis reveals that CmLOX10 and CmLOX13 are members of the type 2 13-LOX subgroup which are known to be involved in biotic and abiotic stress. The two lipoxygenases may play different functions in oriental melon during plant growth and development, CmLOX10 and CmLOX13 enzyme reaction shows that both enzymes produce 13S-hydroperoxides when linoleic acid is used as substrate Cucumis melo var. makuwa
physiological function the isozyme CmLOX10 is involved in biotic and abiotic stress Cucumis melo var. makuwa
physiological function the isozyme CmLOX13 is involved in biotic and abiotic stress Cucumis melo var. makuwa

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
11760
-
 linolenic acid pH 5.0, 45°C, recombinant Hi-tagged enzyme Cucumis melo var. makuwa
17420
-
 linolenic acid pH 5.0, 45°C, recombinant Hi-tagged enzyme Cucumis melo var. makuwa
19390
-
 linoleic acid pH 5.0, 45°C, recombinant Hi-tagged enzyme Cucumis melo var. makuwa
23850
-
 linoleic acid pH 5.0, 45°C, recombinant Hi-tagged enzyme Cucumis melo var. makuwa