Literature summary for 1.13.11.12 extracted from

Pastore, D.; Laus, M.N.; Tozzi, D.; Fogliano, V.; Soccio, M.; Flagella, Z.
New tool to evaluate a comprehensive antioxidant activity in food extracts: Bleaching of 4-nitroso-N,N-dimethylaniline catalyzed by soybean lipoxygenase-1 (2009), J. Agric. Food Chem., 28, 9682-9292.

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Application

Application	Comment	Organism
medicine	the LOX/4-nitroso-N,N-dimethylaniline method is able to highlight high antioxidant activity values in durum wheat grains, as well as synergistic interactions among antioxidants from different extracts, and so provide a more comprehensive and integrated determination of antioxidant activity, which is essential for a total antioxidant activity assessment and may provide a more realistic quantification of food antioxidant effectiveness in preventing diseases	Glycine max

Inhibitors

Inhibitors	Comment	Organism	Structure
alpha-tocopherol	competitive inhibition of the LOX/4-nitroso-N,N-dimethylaniline reaction	Glycine max
apigenin	uncompetitive inhibition of the LOX/4-nitroso-N,N-dimethylaniline reaction	Glycine max
beta-carotene	-	Glycine max
catechin	competitive inhibition of the LOX/4-nitroso-N,N-dimethylaniline reaction	Glycine max
ferulic acid	noncompetitive inhibition of the LOX/4-nitroso-N,N-dimethylaniline reaction	Glycine max
gallic acid	competitive inhibition of the LOX/4-nitroso-N,N-dimethylaniline reaction	Glycine max
glutathione	-	Glycine max
L-ascorbic acid	noncompetitive inhibition of the LOX/4-nitroso-N,N-dimethylaniline reaction	Glycine max
additional information	LOX/4-nitroso-N,N-dimethylaniline reaction is not inhibited by inulin	Glycine max
resveratrol	uncompetitive inhibition of the LOX/4-nitroso-N,N-dimethylaniline reaction	Glycine max
Trolox	noncompetitive inhibition of the LOX/4-nitroso-N,N-dimethylaniline reaction. At physiological pH 7.0 the LOX/4-nitroso-N,N-dimethylaniline assay is more sensitive to trolox inhibition	Glycine max

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.01191	-	linoleic acid	-	Glycine max

Organism

Organism	UniProt	Comment	Textmining
Glycine max	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
linoleic acid + O2	4-nitroso-N,N-dimethylaniline bleaching in the course of linoleate hydroperoxidation by soybean LOX-1	Glycine max	(9Z,11E)-(13S)-13-hydroperoxyoctadeca-9,11-dienoic acid	-	?

Synonyms

Synonyms	Comment	Organism
lipoxygenase-1	-	Glycine max
LOX-1	-	Glycine max

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9	-	-	Glycine max

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
1.1	-	alpha-tocopherol	-	Glycine max
1.7	-	resveratrol	-	Glycine max
3.4	-	apigenin	-	Glycine max
6.7	-	gallic acid	-	Glycine max
7	-	Trolox	-	Glycine max
10.7	-	ferulic acid	-	Glycine max
13.6	-	catechin	-	Glycine max
14	-	L-ascorbic acid	-	Glycine max

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.0078	-	-	Glycine max	beta-carotene
0.0197	-	-	Glycine max	glutathione

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Release 2023.1 (January 2023)