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Literature summary for 1.13.11.1 extracted from
- Cloning, expression and characterization of a mesophilic catechol 1,2-dioxygenase from Rhodococcus ruber OA1 (2017), Biotechnology, 16, 10-18 .
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Rhodococcus ruber |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| K+ | - |
Rhodococcus ruber |  |
| Mg2+ | - |
Rhodococcus ruber | |
| NH4+ | - |
Rhodococcus ruber | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | Mn2+ enhances the activity of the enzyme | Rhodococcus ruber | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| catechol + O2 | Rhodococcus ruber | - |
cis,cis-muconate | - |
? | |
| catechol + O2 | Rhodococcus ruber OA1 | - |
cis,cis-muconate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodococcus ruber | - |
- |
- |
| Rhodococcus ruber OA1 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA resin column chromatography | Rhodococcus ruber |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| catechol + O2 | - |
Rhodococcus ruber | cis,cis-muconate | - |
? | |
| catechol + O2 | - |
Rhodococcus ruber OA1 | cis,cis-muconate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 31700, calculated from amino acid sequence | Rhodococcus ruber |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| C12O | - |
Rhodococcus ruber |
| CatA | - |
Rhodococcus ruber |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
Rhodococcus ruber |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | 45 | more than 50% activity between 15 and 45°C | Rhodococcus ruber |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Rhodococcus ruber |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 8 | more than 50% activity between pH 4.5 and 8.0 | Rhodococcus ruber |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Rhodococcus ruber | calculated from amino acid sequence | - |
4.7 |
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