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Literature summary for 1.13.11.1 extracted from
- High activity catechol 1,2-dioxygenase from Stenotrophomonas maltophilia strain KB2 as a useful tool in cis,cis-muconic acid production (2013), Antonie van Leeuwenhoek, 103, 1297-1307.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, the gene encoding the enzyme is located on the chromosomal DNA, phylogenetic tree | Stenotrophomonas maltophilia |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,2'-dipyridyl | complete inhibition at 2 mM, 99.9% at 1 mM | Stenotrophomonas maltophilia |  |
| 2,4-Dichlorophenol | 26.0% inhibition at 0.1 mM, 29.8% at 0.3 mM | Stenotrophomonas maltophilia | |
| 2-chlorophenol | 31.1% inhibition at 0.1 mM, 17% at 0.3 mM | Stenotrophomonas maltophilia | |
| 2-methylphenol | 24.7% inhibition at 0.1 mM | Stenotrophomonas maltophilia | |
| 3-methylphenol | 20.4% inhibition at 0.1 mM, 32% at 0.3 mM | Stenotrophomonas maltophilia | |
| 4-Chlorophenol | 11% inhibition at 0.1 mM, 13% at 0.3 mM | Stenotrophomonas maltophilia | |
| 4-Methylphenol | 8% inhibition at 0.1 mM, 27.5% at 0.3 mM | Stenotrophomonas maltophilia | |
| catechol | substrate inhibition at over 0.08 mM substrate concentration | Stenotrophomonas maltophilia | |
| EDTA | 99.1 inhibition at 3 mM, 93.7% at 1 mM | Stenotrophomonas maltophilia | |
| Phenanthroline | complete inhibition at 3 mM, 99% at 1 mM | Stenotrophomonas maltophilia | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics, overview | Stenotrophomonas maltophilia | |
| 0.0128 | - |
catechol | pH 8.0, 40°C | Stenotrophomonas maltophilia | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34500 | - |
x * 34500, aabout, sequence calculation | Stenotrophomonas maltophilia |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| catechol + O2 | Stenotrophomonas maltophilia | - |
cis,cis-muconate | - |
? | |
| catechol + O2 | Stenotrophomonas maltophilia KB2 | - |
cis,cis-muconate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Stenotrophomonas maltophilia | A7LBQ5 | - |
- |
| Stenotrophomonas maltophilia KB2 | A7LBQ5 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-methylcatechol + O2 | 11.6% of the activity with catechol | Stenotrophomonas maltophilia | 2-methyl-cis,cis-muconate | - |
? | |
| 3-methylcatechol + O2 | 11.6% of the activity with catechol | Stenotrophomonas maltophilia KB2 | 2-methyl-cis,cis-muconate | - |
? | |
| 4-methylcatechol + O2 | 23.0% of the activity with catechol | Stenotrophomonas maltophilia | 4-methyl-cis,cis-muconate | - |
? | |
| 4-methylcatechol + O2 | 23.0% of the activity with catechol | Stenotrophomonas maltophilia KB2 | 4-methyl-cis,cis-muconate | - |
? | |
| catechol + O2 | - |
Stenotrophomonas maltophilia | cis,cis-muconate | - |
? | |
| catechol + O2 | best substrate | Stenotrophomonas maltophilia | cis,cis-muconate | - |
? | |
| catechol + O2 | - |
Stenotrophomonas maltophilia KB2 | cis,cis-muconate | - |
? | |
| catechol + O2 | best substrate | Stenotrophomonas maltophilia KB2 | cis,cis-muconate | - |
? | |
| additional information | substrate specificity, overview. No activity with 3-chlorocatechol, 4-chlorocatechol, 3,5-dichlorocatechol, 4,5-dichlorocatechol, and hydroquinone | Stenotrophomonas maltophilia | ? | - |
? | |
| additional information | substrate specificity, overview. No activity with 3-chlorocatechol, 4-chlorocatechol, 3,5-dichlorocatechol, 4,5-dichlorocatechol, and hydroquinone | Stenotrophomonas maltophilia KB2 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 34500, aabout, sequence calculation | Stenotrophomonas maltophilia |
| More | three-dimensional structure deduction, molecular modeling, overview | Stenotrophomonas maltophilia |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Stenotrophomonas maltophilia |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | 50 | activity range, profile overview | Stenotrophomonas maltophilia |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
half-life is 3 h | Stenotrophomonas maltophilia |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Stenotrophomonas maltophilia |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 12 | activity range, profile overview | Stenotrophomonas maltophilia |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | three-dimensional structure deduction, molecular modeling, overview | Stenotrophomonas maltophilia |
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